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| 粘质沙雷氏菌测量方法研究 | |
| 引用本文： | 隋志伟,刘晓夏,王晶,傅博强,薛蕾,刘思章,郝林.粘质沙雷氏菌测量方法研究[J].计量学报,2018,39(4):588-592. |
| 作者姓名： | 隋志伟 刘晓夏 王晶 傅博强 薛蕾 刘思章 郝林 |
| 作者单位： | 1.中国计量科学研究院, 北京 100029 2.山西农业大学 食品科学与工程学院, 山西 太谷 030800 3.吉林农业大学 生命科学学院, 吉林 长春 130118 |
| 基金项目： | 国家高技术研究发展计划(2014AA021406);公益性科研院所基本科研业务费专项（AKYS1613） |
| 摘 要： | 粘质沙雷氏菌是生物防护评价的指示菌。对粘质沙雷氏菌营养琼脂平板涂布法进行了方法验证和协同实验验证以及不确定度分析。结果显示,该方法重复性和复现性均较好,室内重复性相对标准偏差为8.93%,室间相对标准偏差为14.95%。经统计分析,营养琼脂平板涂布法相对扩展不确定度为12.60%（k=2）,适用于粘质沙雷氏菌定量测量。 |
| 关 键 词： | 计量学 粘质沙雷氏菌 平板涂布法 营养琼脂 协同实验验证 |
| 收稿时间： | 2016-11-11 |
Study on Measurement Method of Serratia Marcescens |
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| SUI Zhi-wei,LIU Xiao-xia,WANG Jing,FU Bo-qiang,XUE Lei,LIU Si-zhang,HAO Lin.Study on Measurement Method of Serratia Marcescens[J].Acta Metrologica Sinica,2018,39(4):588-592. | |
| Authors: | SUI Zhi-wei LIU Xiao-xia WANG Jing FU Bo-qiang XUE Lei LIU Si-zhang HAO Lin |
| Affiliation: | 1. National Institute of Metrology, Beijing 100029, China 2. College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030800, China 3. College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China |
| Abstract: | Serratia marcescens was traditionally used as microbiology indicator of biological protection evaluation. Nutrient agar tablet coating method for Serratia marcescens were validated, collaborative experimental verified, and uncertainty analyzed. The results showed that nutrient agar tablet coating method had a good repeatability and reproducibility, the interassay relative standard deviations was 8.93%, and external laboratory relative standard deviations was 14.95%. The statistic test showed that the relative expanded uncertainty of nutrient agar tablet coating method was 12.60% (k=2), which could be used for quantitative measurement of Serratia marcescens. |
| Keywords: | metrology Serratia marcescens tablet coating method nutrient agar collaborative experimental verification |
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