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白色链霉菌发酵液中赖氨酸的测定
引用本文:吴琼,刘庆军.白色链霉菌发酵液中赖氨酸的测定[J].中国酿造,2005(11):53-55.
作者姓名:吴琼  刘庆军
作者单位:山东轻工业学院,食品与生物工程学院,山东,济南,250100
摘    要:对天然食品防腐剂ε-聚L-赖氨酸的发酵液中赖氨酸浓度的测定方法进行了研究。结果显示:分光光度法测定白色链霉菌发酵液中的赖氨酸时适用的测量范围是0.075mg/mL~200mg/mL,反应液加热时间20min以上,反应液的最大吸收波长480nm~482nm。发酵液中如果添加脯氨酸对测定结果影响最为明显。

关 键 词:白色链霉菌  L-赖氨酸  测定方法
文章编号:0254-5071(2005)11-0053-03
修稿时间:2005年4月12日

Determining method of lysine content in Streptomyces albulus fermentation broth
WU Qiong,LIU Qing-jun.Determining method of lysine content in Streptomyces albulus fermentation broth[J].China Brewing,2005(11):53-55.
Authors:WU Qiong  LIU Qing-jun
Abstract:Determining method of L-lysine,a natural food antiseptic,in Streptomyces albulus fermentation broth was studied.The results showed that,the determining range of lysine concentration in the broth by spectrophotometer was 0.075-0.200 mg/ml,the suitable reaction time was over 20 min,maximum absorption wavelength was 480-482 nm,and the accession of proline into fermentation broth would have great influence on the result.
Keywords:Streptomyces albulus  L-lysine  determining method
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