| 干海参线粒体的提取与纯化研究 |
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| 引用本文： | 单懿,薛长湖,李兆杰. 干海参线粒体的提取与纯化研究[J]. 食品科学, 2005, 26(10): 111-112 |
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| 作者姓名： | 单懿 薛长湖 李兆杰 |
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| 作者单位： | 中国海洋大学生物学院,山东,青岛,266003 |
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| 摘 要： | 以干海参为原料，采用优化后的溴化十六烷基三甲基铵（CTAB）法提取其线粒体，通过A260/A280值、琼脂糖凝胶电泳和PCR检测，对所得线粒体溶液进行定量，定性分析和综合比较，为干海参的分了生物学研究提供了一套迅速、经济和可靠的技术方案。
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| 关 键 词： | 干海参 线粒体 提取 PCR |
| 文章编号： | 1002-6630（2005）10-0111-0 |
| 收稿时间： | 2004-10-28 |
| 修稿时间： | 2004-10-28 |
Study on Extraction and Purification of Mitochondrial from Dried Sea Cucumbers |
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| SHAN Yi,XUE Chang-hu,LI Zhao-jie. Study on Extraction and Purification of Mitochondrial from Dried Sea Cucumbers[J]. Food Science, 2005, 26(10): 111-112 |
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| Authors: | SHAN Yi XUE Chang-hu LI Zhao-jie |
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| Affiliation: | College of Life Science Ocean University of China, Qingdao 266003, China |
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| Abstract: | The mitochondria of dried sea cucumbers extracted with optimized CTAB method. The quantitative and qualitative analyses through the calculation of A26dA280. Agarose gel electrophoresis and PCR were carded out. The results showed that comparing with other methods, the traditional CTAB method would give quicker and more, economical and dependable results. |
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| Keywords: | dried sea cucumbers mitochondrial extraction PCR |
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