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微藻培养条件优化研究
引用本文:张宇,王志成,张玥,马宁,周红霞.微藻培养条件优化研究[J].应用能源技术,2014(7):15-19.
作者姓名:张宇  王志成  张玥  马宁  周红霞
作者单位:黑龙江省能源环境研究院,哈尔滨150027
摘    要:微藻生物柴油是生物能源领域中很有前景的一个重要方向。本文利用微藻(Chlorella Protothecoides)为研究对象,以BG培养基为底物进行液态培养,培养后用离心干燥后得干微藻。用浊度法和干重法对微藻生长情况进行测定,对微藻培养的最优培养基、接种量和培养温度等进行研究。研究表明微藻培养最适接种量1∶5、初始pH值5.7、培养温度30℃和BG+2g/LC6H12O6培养基。

关 键 词:生物柴油  微藻  液体培养

Optimization Study on Microalgae Culture Conditions
Affiliation:ZHANG Yu, WANG Zhi - cheng, ZHANG Yue, Ma Ning, ZHOU Hong - xia ( Energy and Environmental Research Institute of Heilongjiang Province, Harbin 150027, China)
Abstract:Microalgae biodiesel is an important promising direction in bio- energy field. In this paper,microalgae is used as the research object,and BG medium is used as the substrate to be cultured in liquid,then the dried microalgae is obtained by centrifugation. The microalgae growth is measured with turbidity and dry weight method. The optimal medium for microalgae culture,inoculation and incubation temperature is studied. It is shown in the study that the optimum inoculum is 1∶ 5,initial pH is 5. 7,temperature is 30 ℃ and BG + 2g /LC6H12O6 medium.
Keywords:Biodiesel  Microalgae  Liquid culture
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