| 乳酸乳球菌胞外多糖磷酸化的工艺优化 |
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| 引用本文： | 辛灵莹,潘道东.乳酸乳球菌胞外多糖磷酸化的工艺优化[J].食品科学,2012,33(7):233-236. |
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| 作者姓名： | 辛灵莹 潘道东 |
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| 作者单位： | 1.南京师范大学国家乳品加工技术研发分中心
2.宁波大学海洋学院 |
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| 基金项目： | 浙江省自然科学基金重点项目(Z3110211) |
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| 摘 要： | 采用单因素和正交试验，对乳酸乳球菌胞外多糖的磷酸化工艺进行研究，探讨磷酸盐用量、反应温度、反应时间、反应pH值对乳酸乳球菌胞外多糖最终PO43-接枝量的影响。所得的乳酸乳菌球菌胞外多糖磷酸化的工艺优化条件为：胞外多糖与磷酸盐质量比为6:1、温度90℃、时间4h、pH6.0，此条件下所得PO43-的接枝量为1.639mg/g。
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| 关 键 词： | 乳酸乳球菌 胞外多糖 磷酸化 |
Optimization of Phosphorylation Process for Exopolysaccharide from Lactococcus lactis subsp. lactis |
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| XIN Ling-ying,PAN Dao-dong.Optimization of Phosphorylation Process for Exopolysaccharide from Lactococcus lactis subsp. lactis[J].Food Science,2012,33(7):233-236. |
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| Authors: | XIN Ling-ying PAN Dao-dong |
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| Affiliation: | (1. National Dairy Products Developing Branch Centre, Nanjing Normal University, Nanjing 210097, China；
2. School of Marine Sciences , Ningbo University, Ningbo 315211, China) |
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| Abstract: | One-factor-at-a-time and orthogonal array design methods were employed to optimize the phosphorylation of exopolysaccharide from Lactococcus lactis subsp.lactis.The amount of grafted phosphate was investigated with respect to several process conditions such as the amount of added phosphorylate,reaction temperature,reaction time and pH.The optimal phosphorylation conditions were determined as 1:6 of exopolysaccharide-to-phosphorylate ratio,90 ℃ of reaction temperature,4 h of reaction time and pH 6.0.Under these conditions,the amount of grafted phosphate was up to 1.639 mg/g. |
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| Keywords: | Lactococcus lactis subsp lactis exopolysaccharide phosphorylation |
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