| 紫外分光光度法测定糖蜜中5-羟甲基糠醛含量 |
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| 引用本文： | 冯红伟,扶雄.紫外分光光度法测定糖蜜中5-羟甲基糠醛含量[J].食品工业科技,2010(3). |
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| 作者姓名： | 冯红伟 扶雄 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东,广州,510640 |
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| 摘 要： | 采用紫外分光光度法测定甘蔗糖蜜中5-羟甲基糠醛(5-HMF)含量,以5-羟甲基糠醛为标准品,在284nm波长处对糖蜜样品进行了定量分析。测定结果表明,回归方程为Y=0.02046+0.1145X,R=0.9995,线性范围0～15μg/mL,测定精密度RSD为1.27%,重现性良好。平均回收率为97.91%,回收率RSD为1.67%(n=6),检出限为0.08μg/mL。
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| 关 键 词： | 糖蜜 5-羟甲基糠醛 紫外分光光度法 |
Determination of 5-hydroxymethyl-2-furfural in molasses by ultraviolet spectrophotometry |
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| FENG Hong-wei,FU Xiong.Determination of 5-hydroxymethyl-2-furfural in molasses by ultraviolet spectrophotometry[J].Science and Technology of Food Industry,2010(3). |
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| Authors: | FENG Hong-wei FU Xiong |
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| Affiliation: | FENG Hong-wei,FU Xiong (College of Light Industry , Food Sciences,South China University of Technology,Guangzhou 510640,China) |
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| Abstract: | The content of 5 - hydroxymethyl - 2 - furfural (5 - HMF) in molasses was measured by ultraviolet spectrophotometry(UV) using 5-hydroxymethyl-2-furfural as a standard sample at the wavelength of 284nm.The experimental results demonstrated that the regression equations were:Y = 0.02046 + 0.1145X, R = 0.9995, the linear range was 0～15μg/mL, and the precision of method was 1.27% ; the average recovery rate was 9%91%, RSD was 1.67% ( n =6) ,and the detection limit was 0.08μg/mL. |
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| Keywords: | molasses 5-hydroxymethyl-2-furfural ultraviolet spectrophotometry |
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