Cancer preventive effect of Thai rat-tailed radish (Raphanus sativus L. var. caudatus Alef) |
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| Authors: | Piman Pocasap Natthida Weerapreeyakul Sahapat Barusrux |
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| Affiliation: | 1. Graduate School, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;2. Center for Research and Development of Herbal Health Products (CRD-HHP), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;3. Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand |
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| Abstract: | The cancer preventive effect of Raphanus sativus L. var. caudatus Alef (Thai rat-tailed radish) extract was evaluated against the HCT116 colon cancer cell line. HPLC and GC–MS were used to identify the phytoconstituents in Thai rat-tailed radish extract. Thai rat-tailed radish extract showed high cytotoxicity against HCT116 with an IC50 at 9.42 ± 0.46 μg/ml. Apoptosis induced by Thai rat-tailed radish extract was confirmed (a) by DAPI staining which demonstrated nuclei morphological changes, chromatin condensation and nuclear fragmentation and (b) by Annexin V/PI flow cytometry. GC–MS analysis of Thai rat-tailed radish extract revealed two isothiocyanates; sulforaphane and sulforaphene, whose cytotoxicity and apoptosis induction effect were evident, giving Thai rat-tailed radish extract its anticancer attributes. This is the first report on the cancer preventive effect of Thai rat-tailed radish extract against HCT116 colon cancer cell line, through apoptosis induction. |
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| Keywords: | Cruciferae Sulforaphane Sulforaphene Cytotoxicity Apoptosis induction |
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