基于拉曼光谱的鼻咽癌与正常鼻咽细胞株的分类研究

高空间分辨率的共聚焦显微拉曼光谱技术从分子水平上提供了生物分子成分和结构的指纹性信息。以鼻咽癌细胞株C666-1、CNE2和正常鼻咽细胞株NP69为对象,利用拉曼光谱探索和分析癌细胞株与正常细胞株之间的差别。结果表明:谱峰比I1449/I1657(1.10)可以准确地将鼻咽癌与正常鼻咽细胞株分成两组,这一结论与相关支气管癌组织的报道结果一致;此外,采用主成分分析方法说明3种细胞株的拉曼光谱之间存在统计学差异,并以线性判别分析方法对其进行统计划分,灵敏度和特异性分别达到90%和100%。这些结论可以为拉曼光谱技术用于NPC的临床诊断提供依据,并有可能为鼻咽癌的早期诊断提供有益参考。

Discrimination of Nasopharyngeal Carcinoma and Normal Nasopharyngeal Cell Lines Based on Confocal Raman Microspectroscopy

Raman microspectroscopy can provide molecular-level finger-print information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. Raman spectroscopy of nasopharyngeal carcinoma cell lines C666-1, CNE2 and normal nasopharyngeal cell line NP69 are presented to investigate the differences between them. The ratio of I1449/I1657(1.10) seems to very easily divide tumor and normal cell lines into two groups, and this result is coincident with the existing report about the study of normal and malignant bronchial tissue. Principal component analysis (PCA) and linear discriminant analysis (LDA) are also used to classify different cell lines and achieve an exciting result with a sensitivity and specificity of 90% and 100%, respectively. The results of the work may be helpful to the discrimination of normal and tumor cells, which show that Raman spectroscopy can be one of the diagnostic methods of nasopharyngeal carcinoma and do favor to the early diagnosis.