Batch and Continuous Hydrolysis of Lactose Using P-Galactosidase Immobilized on Gelatin-Cmc

β-Galactosidase enzyme (E.C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate. A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin. The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated. A fluidized-bed reactor with a down flow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.