| 长双歧杆菌多因素连续驯化的研究 |
| |
| 引用本文： | 曹月婷,李玉,李江,姚盟成,路福平. 长双歧杆菌多因素连续驯化的研究[J]. 食品研究与开发, 2011, 32(11): 104-107 |
| |
| 作者姓名： | 曹月婷 李玉 李江 姚盟成 路福平 |
| |
| 作者单位： | 工业发酵微生物教育部重点实验室,工业酶国家工程实验室,天津科技大学生物工程学院,天津300457 |
| |
| 基金项目： | 天津市科技计划项目(10ZCKFNC01700); 国家自然科学基金(青年科学基金项目)(20806060) |
| |
| 摘 要： | 双歧杆菌因对外界环境的耐受性较差,限制了其规模化生产和实际应用。通过对长双歧杆菌进行连续的耐氧、耐酸以及耐胆盐的驯化,使长双歧杆菌对氧、酸以及胆盐的耐受性有了一定的提高,活菌数和产酸性能都有所提高。耐氧驯化时,通过逐渐增加培养基中的氧分压的方法进行;耐酸驯化时,通过逐渐降低培养基的初始pH的方法进行;耐胆盐驯化时,通过逐渐增加培养基初始胆盐含量的方法进行。实验结果表明,耐氧耐酸驯化后的菌株活菌数能达到9.4×108 cfu/mL,为初始菌的1.92倍;耐胆盐驯化后的菌株在胆盐浓度为0.3%时培养也能保持一定的活菌数,而初始菌株不能存活。
|
| 关 键 词： | 长双歧杆菌 耐氧 耐酸 耐胆盐 驯化 |
The Stepwise Domestication of Bifidobacterium Longum for Multiple-stress Tolerance |
| |
| CAO Yue-ting,LI Yu,LI Jiang,YAO Meng-cheng,LU Fu-ping. The Stepwise Domestication of Bifidobacterium Longum for Multiple-stress Tolerance[J]. Food Research and Developent, 2011, 32(11): 104-107 |
| |
| Authors: | CAO Yue-ting LI Yu LI Jiang YAO Meng-cheng LU Fu-ping |
| |
| Affiliation: | CAO Yue-ting,LI Yu,LI Jiang,YAO Meng-cheng,LU Fu-ping(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,National Engineering Laboratory for Industrial Enzymes,The College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
| |
| Abstract: | The production and application of Bifidobacterium is limited because of its poor tolerance to multiple relevant environmental stresses.To improve its tolerance,Bifidobacterium longum was domesticated via sequential exposure to oxygen,decreased pH,and increased bile salt concentrations.First,B.longum cells were domesticated by allowing their growth in stepwisely increased oxygen pressure conditions,followed by next round domestication to achieve acid-resistance by subjecting them to liquid media with gradual... |
| |
| Keywords: | Bifidobacterium longum oxygen-resistance acid-resistance bile salt-resistance domestication |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
