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Estimation of Microbial Populations in Orange Juice by Bioluminescence
Authors:T. R. GRAUMLICH
Affiliation:Author Graumlich is with the Univ. of Florida, Institute of Food &Agricultural Sciences, Citrus Research &Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850.
Abstract:Bioluminescent measurement of microbial ATP was compared to plate counts of orange juice on orange serum agar and plate count agar plus chloramphenicol and chlortetracycline. Bioluminescent values correlated well (r = 0.92) with plate counts of reconstituted juices with active microbial populations. A correlation of 0.58 was found for bioluminescent values to plate counts for orange juice samples immediately after reconstitution from concentrate. Centrifugation and resuspension of samples in 0.1M Tris-HCl buffer, pH 8.0, was utilized to provide adequate pH adjustment and concentration of microorganisms for bioluminescent measurement.
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