饮用水中交链孢霉毒素氯消毒副产物鉴定及其毒性作用评价

目的 研究水中交链孢菌酮酸(TeA)和腾毒素(TEN)在氯消毒过程中的反应动力学特征,对产生的氯消毒副产物(DBPs)进行结构鉴定,并对其消毒副产物的细胞毒性进行初步探索。方法 本研究通过实验室模拟其消毒反应过程,研究其反应动力学特征,利用超高效液相色谱-串联四级杆飞行时间质谱(UPLC-Q-TOF/MS)和核磁共振波谱对反应产物进行结构鉴定并初步评价其毒性。结果 TeA氯消毒反应可生成2种氯消毒副产物DBP-188和DBP-240,而TEN氯代反应很慢未观察到氯消毒副产物的产生。反应动力学试验表明TEN与氯的反应速率随着反应体系中氯浓度和pH值的增加而升高。体外毒性预测分析发现TeA的氯消毒副产物经口毒性、发育毒性和致癌性均高于母体。结论 交链孢霉毒素经过氯消毒会产生氯消毒副产物,反应过程受pH值与氯浓度的影响,氯消毒副产物的预测毒性高于母体,细胞试验证明了TeA氯消毒副产物具有一定的细胞毒性。

Identification and toxicity evaluation of chlorine disinfection by-products of Alternaria toxins in drinking water

Objective To study the reaction kinetics characteristics of tenuazonic acid (TeA) and tentoxin (TEN) in water during chlorination, identify the structure of chlorine disinfection by-products(DBPs), and preliminarily explore the cytotoxicity of disinfection by-products. Methods The disinfection reaction process of TeA and TEN was simulated in the laboratory, and their reaction kinetic characteristics were further investigated. The reaction products were identified by ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and nuclear magnetic resonance spectrum, and the toxicity of TeA and its disinfection by-products were evaluated by toxicity prediction and cytotoxicity. Results TeA produced two kinds of DBPs (DBP-188 and DBP-240) in simulated chlorine disinfection reaction, while the chlorination reaction of TEN was slow, and no DBPs were found. Reaction kinetic studies indicated that the reaction rate of TEN with chlorine increased with increasing chlorine concentration and pH in the reaction system. In vitro toxicity prediction analysis found that the oral toxicity, developmental toxicity and carcinogenicity of the DBPs of TeA were higher than the maternal toxicity. Conclusion Alternaria toxins produced disinfection by-products after chlorination. The reaction process was influenced by the concentration of chlorine and pH.Cellular experiments showed that the DBPs of TeA had some cytotoxicity.