负载二甲酸钾缓释抗菌微球的构建

为避免二甲酸钾(KDF)在酸性环境下分解过快,调节仔猪肠胃道酸碱性和菌落平衡,实现KDF靶向释放抗菌,本研究以可生物降解的壳聚糖(CS)、羧甲基纤维素(CMC)和无机刚性材料P型沸石分子筛(Zeolite P)为载体,负载抗菌药物二甲酸钾制备控释水凝胶微球。发现CS中–NH2与CMC中–COOH离子作用,可形成结构稳定的聚电解质复合物。溶胀率的差异性表明CS/CMC/ZeoliteP水凝胶微球对pH高度敏感。加入ZeoliteP使水凝胶微球在pH1.2保持原有形貌且不被降解破裂。CS/CMC/Zeolite P/KDF抗菌微球的包封率为47.75%,载药率为23.88%,可有效缓释KDF,在p H7.4磷酸盐缓冲溶液中的缓释性比p H1.2更优。CS/CMC/Zeolite P/KDF抗菌微球浓度为96 mg/mL时对大肠杆菌最大抑菌率为83%,有效提高了KDF利用率。

Construction of Sustainable Release Antimicrobial Microspheres Loaded with Potassium Diformate

To prevent potassium diformate(KDF)from decomposing too quickly in an acidic environment,adjust the acidity and alkalinity of the gastrointestinal tract of the piglets and the colony balance,and realize the targeted release of KDF antibacterial,in this study,a kind of hydrogel microspheres were prepared by biodegradable chitosan(CS),carboxymethyl cellulose(CMC),and inorganic rigid material P-type molecular sieve(Zeolite P).Then the antibacterial drug potassium diformate was loaded so as to be controlled to release from this hydrogel microsphere.The results show that–NH2 in CS and–COOH in CMC form a stable polyelectrolyte complex through ion interaction.Swelling rate test indicates that the CS/CMC/Zeolite P hydrogel microspheres display high pH-sensitivity.The addition of Zeolite P allows the hydrogel microspheres to maintain their original morphology at pH1.2 without being degraded or broken.The effective sustained-release KDF in pH7.4 phosphate buffer solution exhibits better sustained-release performance than that in pH1.2.When the concentration of CS/CMC/Zeolite P/KDF antimicrobial microspheres is 96 mg/mL,the maximum bacteriostatic rate of Escherichia coli is 83%,which effectively improves the utilization rate of KDF.