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Modeling of kinetics for the enzymatic hydrolysis of sunflower oil in a high-pressure reactor
Authors:Mateja?Primo?i?,Maja?Habulin,?eljko?Knez  author-information"  >  author-information__contact u-icon-before"  >  mailto:zeljko.knez@uni-mb.si"   title="  zeljko.knez@uni-mb.si"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:(1) Faculty of Chemistry and Chemical Engineering, Smetanova 17, 2000 Maribor, Slovenia
Abstract:This study presents a mathematical model for characterizing the Michaelis-Menten type of sunflower oil hydrolysis, catalyzed by the lipase preparation Lipolase 100T (Novozymes Ays, Bagsvaerd, Denmark). Supercritical carbon dioxide was used as a solvent for this reaction. Computer modeling of the kinetics was done for the enzymatic hydrolysis of sunflower oil in a high-pressure reactor using the MicroMath Scientist® program (Micro-Math Research, St. Louis, MO). On the basis of the experimental results, we presumed the reaction was reversible. The suitability of the model was confirmed statistically with Student's t-test. Good agreement was found between the experimental and calculated values for the concentrations of oleic and linoleic acids.
Keywords:Hydrolysis  lipase  Michaelis-Menten kinetics  modeling of enzyme kinetics  sunflower oil  supercritical  supercritical carbon dioxide
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