离子色谱法同时测定牛奶及其制品中的亚硝酸盐、硝酸盐、硫酸盐和硫氰酸盐

建立了离子色谱同时测定牛奶及其制品中亚硝酸盐、硝酸盐、硫酸盐和硫氰酸盐的方法.该方法采用三氯乙酸-乙醇对样品进行萃取并离心,上清液经C18固相萃取小柱净化后,利用阴离子色谱柱分离、检测.试验结果表明:该方法的线性范围和相关系数为0.02～5.0 μg/mL和0.9992,检出限为0.005～0.02 mg/kg;相对标...

Simultaneous Determination of Nitrite,Nitrate,Sulfate and Thiocyanide in Milk and its Products by Ion Chromatography

An ion chromatography was developed for the simultaneous quantitative analysis of nitrite,nitrate,sulfate and thiocyanide in milk and its products.Samples were ultrasonically extracted with the mixture of ethanol and trichloroacetic acid solution,purified by C18 SPE column and then analyzed by IC using an anion column.Under the optimal conditions,the limits of detection(LODs,S/N=3) and the relative standard deviation(RSDs,N=6) for the target compounds were in the range of 0.01～0.02 mg/kg and 0.87～2.6%,respectively.The calibration curve was linear in the concentration range of 0.02～5.0 ?g/ml with the regression coefficient of 0.9992.To validate the proposed method,the real samples were analyzed and the recoveries of 87.8～100.4% were obtained.The proposed method was rapid,effective,sensitive,accurate and applied to milk and its products.