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青梅酱中的有机酸成分分析
引用本文:林耀盛,杨春英,陈智毅,钟炜雄,刘学铭.青梅酱中的有机酸成分分析[J].现代食品科技,2011(9):1150-1153.
作者姓名:林耀盛  杨春英  陈智毅  钟炜雄  刘学铭
作者单位:广东省农业科学院蚕业与农产品加工研究所省部共建国家重点实验室培育基地-广东省农产品加工重点实验室;陆河县伟能食品有限公司;
基金项目:广东省科技计划项目(2009B020410003)
摘    要:分析了青梅酱的可溶性固形物、总酸、还原糖等主要成分,并采用高效液相色谱法对其中的草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸及琥珀酸等7种有机酸进行了测定.有机酸分析采用的色谱柱为Zorbax SB-Aq C18(4.6x250 mm,5μm),流动相为0.01 mol/L KH2 PO4(pH 2.8),流速为1mL/m...

关 键 词:青梅酱  有机酸  总酸  高效液相色谱法

Study of Main Composition and Organic Acid in Greengage sauce
LIN Yao-sheng,YANG Chun-ying CHEN zhiyu,ZHONG weixiong,LIU Xue-ming.Study of Main Composition and Organic Acid in Greengage sauce[J].Modern Food Science & Technology,2011(9):1150-1153.
Authors:LIN Yao-sheng  YANG Chun-ying CHEN zhiyu  ZHONG weixiong  LIU Xue-ming
Affiliation:LIN Yao-sheng1,YANG Chun-ying1 CHEN zhiyu1,ZHONG weixiong2,LIU Xue-ming1(1.The Sericulture & Agri-Food Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China)(2.Luhe Wei Neng Shi Pin Co.,Ltd,Luhe 510610,China)
Abstract:The contents of soluble solids,total acids and reducing sugars in Prunus mume sauce were analyzed,and the profile of organic acids,including oxalic acid,tartaric acid,malic acid,lactic acid,acetic acid,citric acid and succinic acid,was investigated with HPLC.The chromatographic column used was Zorbax SB-Aq C18(4.6×250 mm,5 μm).The conditions were as follows: mobile phase 0.01 mol·L-1 KH2PO4(pH 2.8),flow rate 1 mL/min,column temperature 30 ℃ and detection wavelength 215 nm.The recovery rate of organic acid was from 121.0% to 75.4%.The relative standard deviation was in the range of 0.78% to 2.12%.The detection limit ranged from 0.022~1.290 μg/mL.The results showed that the content of soluble solids,total acids and total reducing sugars were 59.45%,12.29% and 35.70%,respectively.Seven kinds of organic acids were detected in Prunus mume sauce,which were comparable with the contents of organic acids in Prunus mume fruit.
Keywords:Prunus mume sauce  organic acids  total acids  High performance liquid chromatography  
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