| 一株对苯二甲酸降解菌的鉴定及其降解特性 |
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| 引用本文： | 冯杨阳,陈俊,刘波,陈英文,郑国洋,陆建华,沈树宝. 一株对苯二甲酸降解菌的鉴定及其降解特性[J]. 化工学报, 2006, 57(8): 1968-1973 |
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| 作者姓名： | 冯杨阳 陈俊 刘波 陈英文 郑国洋 陆建华 沈树宝 |
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| 作者单位： | 南京工业大学工业生物技术研究所，江苏 南京 210009;中国石油化工股份有限公司扬子石油化工责任有限公司，江苏 南京 210048 ; |
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| 基金项目： | 科技部科研项目 , 国家高技术研究发展计划(863计划) , 江苏省社会发展基金 |
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| 摘 要： | 从扬子石油化工责任有限公司废水处理装置的污泥池中分离、筛选得到了一株高效降解对苯二甲酸（PTA）的菌株，PA-18.采用PCR扩增获得该菌16SrDNA片段，核苷酸序列分析结果表明：该菌株的16SrDNA的核苷酸序列与多株假单胞菌（Pseudomonas sp.）的同源性为99%，在细菌系统发育分类学上，该菌株属于假单胞属.在温度为37℃、pH 7.0、OD660 nm2.0条件下，反应24 h，此菌对PTA的降解率大于95 %.同时对于实际的PTA废水也有很好的处理效果，CODCr去除率高达85%.在好氧降解作用下，PTA降解的主要中间物被确定为原儿茶酸.
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| 关 键 词： | 对苯二甲酸 16SrDNA 假单胞菌 鉴定 |
| 文章编号： | 0438-1157（2006）08-1968-06 |
| 收稿时间： | 2005-12-23 |
| 修稿时间： | 2005-12-232006-02-27 |
Identification and characterization of a PTA-degrading bacterial strain |
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| FENG Yangyang,CHEN Jun,LIU Bo,CHEN Yingwen,ZHENG Guoyang,LU Jianhua,SHEN Shubao. Identification and characterization of a PTA-degrading bacterial strain[J]. Journal of Chemical Industry and Engineering(China), 2006, 57(8): 1968-1973 |
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| Authors: | FENG Yangyang CHEN Jun LIU Bo CHEN Yingwen ZHENG Guoyang LU Jianhua SHEN Shubao |
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| Affiliation: | 1.Industrial Biotechnology Research Center, Nanjing University of Technology, Nanjing 210009, Jiangsu, China; 2.Yangtze Petrochemical Company, SINOPEC, Nanjing 210048, Jiangsu, China |
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| Abstract: | A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company.The 16SrDNA fragment was amplified from strain PA-18 by PCR，and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp..In the phylogenetic framework of bacterial classification，PA-18 belongs to Pseudomonas sp..Under the condition of temperature 37℃，pH 7.0 and OD660 nm2.0，the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h.Meanwhile，this strain also could treat PTA wastewater with CODCr removal of 85%.p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state. |
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| Keywords: | p-phthalic acid 16SrDNA Pseudomonas sp. identification |
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