Validation of a UV Spectrophotometric Method for Determination of Proteolytic Activity of Enzymatic Detergents |
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| Authors: | Leonardo S. Lopes Filipe S. Q. Silva Adriana S. Silva Isabella F. Delgado |
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| Affiliation: | 1. +55‐21‐38655128+55‐21‐22900915;2. Departamento de Química, Instituto Nacional de Controle de Qualidade em Saúde, , CEP 21040‐900 Rio de Janeiro, Brazil;3. Programa de Pós‐gradua??o em Vigilancia Sanitária, Instituto Nacional de Controle de Qualidade em Saúde, , CEP 21040‐900 Rio de Janeiro, Brazil;4. Instituto Nacional de Controle de Qualidade em Saúde, , CEP 21040‐900 Rio de Janeiro, Brazil |
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| Abstract: | Enzymatic detergents are widely used in health services. These products should be controlled to ensure their activity. In this paper, a UV spectrophotometric method for the determination of proteolytic activity in enzymatic detergents was validated. An artificial substrate (azocasein) was used to quantify the enzyme activity at 340 nm. The results showed that the method was satisfactory in respect to its specificity, linearity, precision, accuracy and robustness. This method fulfills both ICH’s and Brazilian criteria, demonstrating its suitability for routine analysis as well as its potential application for regulatory purposes. |
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| Keywords: | UV spectrophotometry Enzyme Detergent |
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