高效液相测定沙棘果皮中熊果酸和齐墩果酸的含量

建立沙棘果皮中齐墩果酸、熊果酸的含量测定方法。采用高效液相色谱法,Nova-Pack型C18柱,乙腈-甲醇-水-醋酸铵（65∶20∶15∶0.5）为流动相,检测波长为210nm;流速0.6mL/min。熊果酸回归方程为y=74.0x＋9.12,r=0.9995,线性范围0.11～10.64μg/mL,齐墩果酸回归方程为y=61.6x＋7.40,r=0.9992,线性范围0.12～12.36μg/mL。沙棘果皮中熊果酸和齐墩果酸的平均含量为1.187mg/g和0.352mg/g,平均回收率为100.3%、100.5%（n=3）,RSD分别为1.47%、1.50%（n=3）。该方法可同时测定沙棘果皮中齐墩果酸和熊果酸的含量,可用于药材质量控制。

Simultaneous Determination of Ursolic acid and Oleanolic acid in Hippophae rhamnoides with HPLC

To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolie acid in pericarp of Hippophae rhamnoides. Nova-pak C18 column （150 mm × 3.9 mm, 5 μm） was used. The mobile phase consists of acetonitrile, methanol, H2O, ammonium acetate （65 ： 20 ： 15 ： 0. 5）, and the flow rate was 0.6 mL/min. Oleanolic acid and ursolie acid were detected at 210 nm. There is an good excellent linearity between in the range of 0.11～10.64 μg/mL and 0. 12～ 12. 36 μg/mL with the regression equation of y=74.0 x＋9. 12 and y=61.6 x＋7. 40 for ursolic and oleanolec, respectively. The average content of ursolic acid and oleanolec acid in pericarp of Hippophae rhamnoides was 1. 187 mg/g and 0. 352 mg/gand the average recoveries was 100.3 % and 100.5% （n=3）with RSD of 1.47%and 1.50%（n=3）, respectively. The method could determine ursolic and oleanolic acid in pericarp of Hippophae rhamnoides. It could be used in the quality control of the medicinal herbs.