水溶性C60衍生物的光致细胞膜毒性的动态研究

用激光共聚焦扫描显微镜研究了在低功率（≤1mW)激光下水溶性C60丙二酸衍生物（DMA C60）对HeLa细胞细胞膜的光诱导损伤及该过程中细胞内钙离子浓度（[Ca^2 ]i)的变化，发现DMA C60对HeLa细胞细胞膜的光诱导损伤过程在低浓度下（≥1μM）即可发生，而且有浓度依赖性。值得注意的是，实验发现在膜损伤发生前伴随细胞内钙离子浓度的瞬时性升高。这些结果直接证实细胞膜是C60衍生物的光敏作用靶点，并提示钙信号可能参与这一过程。

Dynamic Research of Photo-induced Toxicity against Cell Membrane by a Water-soluble C60 Derivative

By using confocal laser scanning microscope, the photo-induced damage to cell m embrane of HeLa cells by dimalonic acid C60 (DMA C60) was investigat ed in the presence of low powerful laser source (P≤1mW), as well as the c hange of intracellular calcium concentration during the damage. The data showed that t he photo-induced toxicity against cell membrane activity occurred even at the p resence of low concentration of DMA C60 (≥1μ mol/L), and the damage was dose -dependent. Furthermore, it was found that the transient increase of intracellu lar calcium concentration took place before the damage of cell membrane. These r esults demonstrated that cell membrane was a target of photosensitive action pro duced by C60 derivative, and implied that calcium signaling was probably i nvolved in the photosensitive action.