| 精炼糖厂离交洗脱液色素黄酮总酚含量的测定及抗氧化物活性的研究 |
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| 引用本文： | 钟映萍,邓广华,蚁细苗,谭文兴,李雨虹,蔡碧云.精炼糖厂离交洗脱液色素黄酮总酚含量的测定及抗氧化物活性的研究[J].甘蔗糖业,2014(6):49-53. |
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| 作者姓名： | 钟映萍 邓广华 蚁细苗 谭文兴 李雨虹 蔡碧云 |
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| 作者单位： | 1. 广州甘蔗糖业研究所 广东省甘蔗改良与生物炼制重点实验室,广东广州,510316
2. 广州市华侨糖厂,广东广州,510760 |
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| 基金项目： | 广州市科技计划项目资助 |
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| 摘 要： | 以抗坏血酸和茶多酚为阳性对照，测定离交洗脱液色素的总黄酮及总多酚含量，并采用1，1-二苯基。2．三硝基苯肼（DPPH·）、（2，2’-连氨-（3-乙基苯并噻唑林-6-磺酸）二氨盐）自由基（ABTS＋·）、铁离子还原／抗氧化能力（FRAP）测定法，评价离交洗脱液色素在质量浓度为0．005～1mg／mL范围内的DPPH-自由基清除能力、ABTS＋·自由基清除能力、铁离子的还原／抗氧化能力，考察抗氧化物总黄酮及总多酚含量与抗氧化能力的相关性。
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| 关 键 词： | 离交洗脱液 抗氧化活性 DPPH ABTS FRAP 总黄酮 总多酚 |
Determination of Total Phenol Content and Total Flavone in the Eluent of Refined Sugar Mill and the Study of Antioxidant Activity |
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| ZHONG Ying-ping,DENG Guang-hua,YI Xi-miao,TAN Wen-xing,LI Yu-hong,CAI Bi-yun.Determination of Total Phenol Content and Total Flavone in the Eluent of Refined Sugar Mill and the Study of Antioxidant Activity[J].Sugarcane and Canesugar,2014(6):49-53. |
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| Authors: | ZHONG Ying-ping DENG Guang-hua YI Xi-miao TAN Wen-xing LI Yu-hong CAI Bi-yun |
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| Affiliation: | ZHONG Ying-ping, DENG Guang-hua, YI Xi-miao, TAN Wen-xing, LI Yu-hong, CAI Bi-yun (1Guangzhou Sugarcane Industry Research Institute/Guangdong Key Lab of Sugarcane Improvement ＆ Biorefinery, Guangzhou 510316; 2Guangzhou Huaqiao Sugar Factory, Guangzhou 510760) |
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| Abstract: | In this article we used ascorbic acid and polyphenols as a positive control to measure total flavonoids and total polyphenol content of the cross eluent pigments. The 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl （DPPH-）, 2,2＇-Azinobis-（3-ethylbenzthiazoline-6-sulphonate） （ABTS＋.）, ferric ion reducing / antioxidant capacity （FRAP） assay was used to evaluate DPPH. radical scavenging, ABTS＋- radical scavenging ability, ferric ion reducing / antioxidant capacity of the cross wash deliquoring concentration of pigment in the 0.005-1 mg/mL range to investigate the correlation between total flavonoids, total polyphenol content and antioxidant capacity. |
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| Keywords: | Ion exchange wastewater Antioxidant activity DPPH ABTS FRAP Flavonoids Total phenols |
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