| 碳源和氮源对纤维素木聚糖复合水解酶合成的影响 |
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| 引用本文： | 吕晓霞,李锁柱,王玉花,王凤翔.碳源和氮源对纤维素木聚糖复合水解酶合成的影响[J].辽宁化工,2010,39(2):128-130,133. |
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| 作者姓名： | 吕晓霞 李锁柱 王玉花 王凤翔 |
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| 作者单位： | 1. 曲阜师范大学化学与化工学院,山东,曲阜,273165
2. 下沙恒升化工有限公司,浙江,杭州,310018 |
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| 摘 要： | 以富含纤维素和半纤维素的农副产物玉米芯为发酵底物,以实验室中绿色木霉为产酶菌种纤维素,进行木聚糖复合水解酶的诱导合成研究。结果表明:碳源和氮源种类、浓度及碳氮比等因素对纤维素酶和木聚糖酶的合成都有影响。合成的最佳条件是以经过1%NaOH预处理的玉米芯为主要碳源和诱导物,碳源浓度为20 g/L,利用蛋白胨+硫酸铵+尿素为复合氮源,控制碳氮比为5.6。
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| 关 键 词： | 玉米芯 纤维素酶 木聚糖酶 |
Effect of Carbon Source and Nitrogen Source on Synthesis of Cellulase and Xylanase |
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| LV Xiao-xia,LI Suo-zhu,WANG Yu-hua,WANG Feng-xiang.Effect of Carbon Source and Nitrogen Source on Synthesis of Cellulase and Xylanase[J].Liaoning Chemical Industry,2010,39(2):128-130,133. |
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| Authors: | LV Xiao-xia LI Suo-zhu WANG Yu-hua WANG Feng-xiang |
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| Affiliation: | 1.School of Chemistry and Chemical Engineering/a>;Qufu Normal University/a>;Qvfu 273165/a>;China/a>;2.Xiasha Hengsheng Chemical Co.Ltd/a>;Hangzhou 310018/a>;China |
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| Abstract: | Synthesis of cellulase and xylanase with pretreated corn-cob as carbon source by Trichoderma viride was studied.The result shows that the kind of carbon source,the kind of nitrogen source and the C/N etc.all have some effects on synthesis of cellulase and xylanase.The best synthetic conditions are asfollows: using 20 g/L corn-cob pretreated by 1% NaOH as carbon source and the peptone + annomium sulfate + urea as nitrogen source and regulating C/N 5.6. |
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| Keywords: | Corn-cob Cellulase Xylanase |
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