阵列细胞微培养腔的设计与制造

采用光刻成型技术、"多次光刻、一次显影"工艺以及热键合工艺制备了基于三层、具有阀控开关和浓度梯度的PDMS微阵列细胞微培养腔,重点研究了浓度梯度、细胞培养腔结构的设计.研究表明浓度梯度的形成主要受芯片管道结构影响;在低雷诺数、被动混合方式情况下,采用简单结构进行混合的效率会很低;在本课题中,将瓦片状结构与管道复合起来提高混合效率,实验结果表明:即使在高流速情况下,也能得很高的混合效率并使各管道间浓度呈很好的线性分布;同时,对细胞培养腔的结构进行了设计;此外,将有限元分析软件COMSOL应用到混合管道及培养腔内流体、流态进行了分析,进一步利用分析结果优化了结构设计.

The design and fabrication of array based cell culture chamber

The fabrication of the amicro chamber array for cell culture,Array based on micro cell culture chamber was successfully completed by Polymer-MEMS technology,employing three SU-8 layers without development after exposure until the end of the process and the micro cell culture chip was equipped with valves utilized to switch switched the channels for cell infusion on and off and could generate different concentrations.In this paper,formation of concentration and design of structures of cell culture chamber we...