The immune response of cattle, persistently infected with noncytopathic BVDV, after superinfection with antigenically semi-homologous cytopathic BVDV

The present study was undertaken to investigate firing patterns, locations, and projections to the phrenic motor nucleus of respiratory neurons in medullary raphe nuclei of rat. Experiments were performed on spontaneously breathing rats anesthetized with sodium pentobarbital. Extracellular spikes of single respiratory neurons were explored in midline medullary tegmentum. A total of 107 respiratory neurons was recorded in the raphe magnus, obscurus and pallidus. They were classified into the following eight types based on the relation of their firing patterns to the phase of respiration: (1) Inspiratory (I) throughout (n = 42); (2) I-late (n = 9); (3) I-decrementing (n = 1); (4) Pre-I (n = 2); (5) I-frequency modulated (n = 13); (6) Post-I (n = 12); (7) Expiratory (E) (n = 23) and (8) E-frequency modulated neurons (n = 5). Twenty of the 45 respiratory neurons examined were antidromically activated from the phrenic motor nucleus at the C4 spinal level with thresholds of 2-58 microA and latencies of 0.4-2.4 ms. Among the 20 neurons, 11 neurons were I-throughout, five were I-frequency modulated and four were E neurons. These results suggest that there is a population of neurons in the medullary raphe nuclei that projects to the phrenic motor nucleus at the C4 spinal level. It is possible that this projection may, in part, mediate the control of the diaphragmatic muscle motor neurons located in the C4 segments.