2000—2018年福建省单核细胞增生李斯特菌分离株血清型及脉冲场凝胶电泳分型

目的 分析福建省食品、临床病例和环境中单核细胞增生李斯特菌分离株的血清型和脉冲场凝胶电泳(PFGE)分型,为食源性疾病的暴发识别和溯源提供参考依据。方法 采用多重聚合酶链式反应(PCR)血清学分型、免疫血清凝集和PFGE方法对2000—2018年分离的单核细胞增生李斯特菌进行分型。结果 117株单核细胞增生李斯特菌分为4组血清型,包括1/2a(3a)、1/2b(3b)、1/2c(3c)和4b(4d,4e),占比分别为67.5%(79/117)、23.1%(27/117)、5.1%(6/117)和4.3%(5/117)。9株病例分离株血清型为1/2a(6株)、4b(2株)和1/2b(1株)。采用Asc I限制性内切酶将117株单核细胞增生李斯特菌分为83种不同PFGE型别,其中10株具有独特单一的型别。9株病例分离株分为8种不同PFGE型别。结论 福建省食品和病例中分离的单核细胞增生李斯特菌,1/2a血清型占主导地位,4b血清型应引起关注。

Analysis of serotypes and pulsed field gel electrophoresis molecular type of Listeria monocytogenes in Fujian Province from 2000 to 2018

Objective To analyze the serotypes and molecular type of Listeria monocytogenes strains isolated from food, clinical case and environment in Fujian Province, so as to provide reference for the outbreak identification and traceability of foodborne diseases. Methods Using multiplex polymerase chain reaction(PCR) serotyping, immune serum agglutination and pulsed field gel electrophoresis (PFGE) to classify the strains. Results The 117 strains of Listeria monocytogenes strains were divided into 4 PCR serotypes, 67.5%(79/117) of which were 1/2a(3a), 23.1%(27/117) were 1/2b(3b), 5.1%(6/117) were 1/2c(3c) and 4.3%(5/117) were 4b(4d,4e). Among the 9 strains isolated from cases, 6 strains were 1/2a, 2 strains were 4b and 1 strain was 1/2b. 117 Listeria monocytogenes strains were divided into 83 different PFGE types by Asc I restriction endonucleases, and 10 strains of which had unique and single types. Nine clinical case isolates were divided into 8 different PFGE types. Conclusion 1/2a serotype was dominant in Listeria monocytogenes isolated from food and clinical specimens in Fujian Province, and 4b serotype should be concerned.