鸡蛋卵转铁蛋白过敏原的体外模拟消化特性

采用体外静态消化模式,分别模拟婴幼儿和成年人的胃液、小肠液以及小肠刷状缘膜酶消化鸡卵转铁蛋白,利用Tricine-SDS-PAGE和MALDI-TOF-MS对消化产物进行分析,研究鸡蛋蛋清过敏原卵转铁蛋白的消化特性.结果表明:婴幼儿和成年人的模拟胃液、小肠液以及小肠刷状缘膜酶都能够消化卵转铁蛋白.在模拟婴幼儿消化体系中...

In Vitro Simulation of Digestive Characteristics of Ovotransferrin in Egg White

Static digestion mode in vitro was used to digest egg ovotransferrin by the simulated gastric juice,small intestine fluid and small intestinal brush border membrane enzyme in infants and adults,respectively.Tricine-SDS-PAGE and MALDI-TOF-MS were used to analyze the digestion products at all stages to define the digestibility of ovotransferrin.It was shown that ovotransferrin could be digested by the simulated gastric juice,small intestine fluid and small intestinal brush border membrane enzyme in infants and adults.Regarding to the digestion in simulated gastrointestinal fluids in infants,the molecular weight of gastric digestion products were 5-15 ku and 30-50 ku.The small intestinal digestion products were 0.5-10 ku.At last,0.5-0.7 ku peptides were produced by small intestinal brush border membrane enzyme.The molecular weight of the digested ovotransferrin by the simulated gastric fluid in adult was the same as that of infants,while 0.5-0.8 ku peptides were mainly produced in intestinal digestion.However,only 0.5-0.7 ku peptides were generated after further digestion by small intestinal brush border membrane enzyme.This work preliminarily defined the characteristics of in vitro gastrointestinal digestion of ovotransferrin,providing some experimental support for better understanding that ovotransferrin is more likely to cause egg allergy in infants.