主动自发气调对枝孢菌的抑制效果

为研究主动自发气调对霉菌的抑制效果,以枝孢菌为试材,研究O2/CO2主动自发气调对枝孢菌的抑菌效果。本试验设计初始O2/CO2体积比例分别为60%O2+40%CO2、70%O2+30%CO2、80%O2+20%CO2、90%O2+10%CO2和自然空气(ck)5个处理,在(28±0.5)℃处理条件下对枝孢菌的菌落直径、孢子量、细胞膜渗透性、细胞内容物释放量、处理后枝孢霉生长曲线及多聚半乳糖醛酸酶(PG)、多聚甲基半乳糖醛酸酶(PMG)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性进行定期测定。结果表明,O2/CO2主动自发气调处理显著抑制菌落扩展速率及孢子生成速率,降低枝孢菌SOD、CAT、PG和PMG活性,并破坏枝孢菌细胞膜完整性,使其孢子内容物释放量及电导率增大,且抑菌效果随着CO2比例的提高而显著增强。本研究结果为果蔬贮藏期间霉菌病害控制提供了理论依据。

Inhibitory Effect of Active Modified Atmospheres on Cladosporium link

The objective of this study is to investigate the effect of active modified atmospheres(Active MAP)on the inhibition of mold.In this study,Cladosporium link was used as test material,and the initial gas proportion of O2/CO2 including 60%O2+40%CO2,70%O2+30%CO2,80%O2+20%CO2,90%O2+10%CO2,and natural air(ck)were employed.During storage at(28±0.5)℃,the colony diameter,spore amount,cell membrane permeability,release of cellular contents,and subsequent effects of growth curve,polygalacturonase(PG),polymethylgalacturonases(PMG),superoxide dismutase(SOD),and catalase(CAT)were periodically measured.The results showed that O2/CO2 active MAP significantly inhibited the colony expansion rate and sporulation rate of Cladosporium link,reduced the activity of SOD,CAT,PG,and PMG,destroyed the cell membrane integrity,increased the cell membrane permeability and release of cellular contents.Furthermore,the antibacterial effect was significantly enhanced along with the increase of CO2 ratio.This study provides a theoretical basis for mold control during fruits and vegetables storage.