土田七中化学成分的分离与鉴定

为研究土田七的化学成分,本文利用多种色谱分离技术对土田七进行分离纯化,采用质谱和核磁共振波谱鉴定化合物结构,从土田七75%乙醇提取物的乙酸乙酯部位和正己烷部位中,分离鉴定了11个化合物,分别鉴定为stigmastern(1)、2-hydroxy-1,2-bis5-(hydroxymethyl)-2-furanyl]ethanone(2)、(Z)-2-acetoxy-5-ethylidene-2-methyl-3-methylenehexanedioic acid(3)、hexanedioic acid,2-(acetyloxy)-5-ethyl-idene-2,3-dimethyl-, R-R*,S*-(Z)]]-(9CI)(4)、hexanedioic acid, 5-ethylidene-2-hydroxy-2,3-dimethyl-, R-R*,S*-(Z)]]-(9CI)(5)、1,4-dihydroxy-2-methylbenzene(6)、2-ethyl-2-butenoic acid(7)、2-furoic acid(8)、(2E)-3-(4-hydroxyphenyl)-2-propenoic acid(9)、(2Z)-3-(4-hydroxyphenyl)-2-propenoic acid(10)、senecionan-11,16-dione(11)。其中3为新化合物,其余化合物为已知化合物。

Isolation and Identification of Chemical Constituents from Stahlianthus Involucratus

To study the chemical constituents of Stahlianthus involucratus, different column chromatographic techniques were used to separate and purify the chemical constituents from the ethyl acetate fraction and n-hexane fraction of Stahlianthus involucratus, and their structures were elucidated by mass spectrometry and NMR. Eleven compounds were separated and purified, which were identified as: stigmastern (1), 2-hydroxy-1,2-bis5- (hydroxymethyl)-2-furanyl]ethenone (2), (Z)-2-acetoxy-5-ethylidene-2-methyl-3-methylenehexanedioic acid (3), hexanedioic acid, 2-(acetyloxy)-5-ethylidene-2,3-dimethyl-, R-R*,S*-(Z)]]-(9CI) (4), hexanedioic acid, 5-ethylidene-2-hydroxy-2,3-dimethyl-, R-R*,S*-(Z)]]-(9CI) (5), 1,4-dihydroxy-2-methylbenzene (6), 2-ethyl-2-butenoic acid (7), 2-furoic acid (8), (2E)-3-(4-hydroxyphenyl)-2-propenoic acid (9), (2Z)-3-(4-hydroxyphenyl)-2-propenoic acid (10), senecionan-11,16-dione (11). Among these compounds, compound 3 was a new natural product and the remaining compounds were known compounds.