| 重组大肠杆菌全细胞用于苯乙酮酸的绿色生物合成 |
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| 引用本文： | 唐存多,史红玲,和子涵,丁朋举,焦铸锦,阚云超,姚伦广. 重组大肠杆菌全细胞用于苯乙酮酸的绿色生物合成[J]. 化工学报, 2018, 69(6): 2627-2631. DOI: 10.11949/j.issn.0438-1157.20171414 |
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| 作者姓名： | 唐存多 史红玲 和子涵 丁朋举 焦铸锦 阚云超 姚伦广 |
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| 作者单位： | 1. 南阳师范学院昆虫生物反应器河南省工程实验室和河南省南水北调中线水源区生态安全重点实验室, 河南 南阳 473061;2. 华东理工大学生物反应器工程国家重点实验室, 上海 200237 |
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| 基金项目： | 河南省科技攻关项目（162102210116）；南阳师范学院青年项目（17359）；河南省科研服务平台专项资助（2016151）；河南省南水北调中线水源区水生态安全创新型科技团队专项（17454）；河南省科技厅基础与前沿项目（162300410137）；河南省高等学校重点科研项目（16A2100347）。 |
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| 摘 要： | 苯乙酮酸是化学合成中重要的合成砌块，可用于合成多种药物中间体，探索苯乙酮酸的绿色合成工艺具有重要的意义。以包含D-扁桃酸脱氢酶LhDMDH编码基因的重组大肠杆菌全细胞为催化剂，考察了它在无辅酶和辅底物添加的条件下对D-扁桃酸生物转化的效果，并对催化产物进行了纯化和鉴定。结果表明，本研究成功实现了在无辅酶和辅底物添加条件下苯乙酮酸的生物合成，产物的得率和纯度分别为45%和99%左右。成果也为外消旋扁桃酸的手性拆分及苯乙酮酸的生物合成奠定了基础。
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| 关 键 词： | 扁桃酸脱氢酶 苯乙酮酸 全细胞催化 绿色化学 生物转化 |
| 收稿时间： | 2017-10-24 |
| 修稿时间： | 2017-11-22 |
Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells |
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| TANG Cunduo,SHI Hongling,HE Zihan,DING Pengju,JIAO Zhujin,KAN Yunchao,YAO Lunguang. Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells[J]. Journal of Chemical Industry and Engineering(China), 2018, 69(6): 2627-2631. DOI: 10.11949/j.issn.0438-1157.20171414 |
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| Authors: | TANG Cunduo SHI Hongling HE Zihan DING Pengju JIAO Zhujin KAN Yunchao YAO Lunguang |
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| Affiliation: | 1. Henan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, Nanyang 473061, Henan, China;2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | Phenylglyoxylic acid (PGA) is key building block in the chemical synthesis, which could be used to synthesize a variety of important pharmaceutical intermediate, thus exploiting the green synthesis process of phenylacetone acid has significant economic value. The recombinant Escherichia coli whole cells containing the encoding gene of LhDMDH was used as catalyst in this study. The biotransformation of D-mandelic acid was researched under the condition of no coenzyme and cosubstrate addition. Subsequently, the transformed products were purified and identified. These results indicated that biosynthesis of phenylglyoxylic acid was realized under the condition of no coenzyme and cosubstrate addition. The yield and purity of PGA were 45% and 99%, respectively. In addition, this study established a solid foundation for chiral resolution of racemic mandelic acid and the biosynthesis of PGA. |
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| Keywords: | mandelate dehydrogenase phenylglyoxylic acid whole cell catalysis green chemistry biotransformation |
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