| 氧化石墨烯固定化α-淀粉酶 |
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| 引用本文： | 张晗,华绍烽,骆辉,张磊,范云场,郭丰哲,韦冬悦,张美金. 氧化石墨烯固定化α-淀粉酶[J]. 工业催化, 2018, 26(10): 92-96. DOI: 10.3969/j.issn.1008-1143.2018.10.013 |
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| 作者姓名： | 张晗 华绍烽 骆辉 张磊 范云场 郭丰哲 韦冬悦 张美金 |
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| 作者单位： | 河南理工大学化学化工学院,河南 焦作 454000 |
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| 基金项目： | 河南省科技厅科技攻关项目(142102210049); 河南省教育厅科学技术重点研究项目(14B150026); 化工资源有效利用国家重点实验室开放课题(CRE-2017-C-305); 河南理工大学创新型科研团队(T2018-3) |
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| 摘 要： | 以石墨粉为原料,使用改进Hummer法制备氧化石墨烯,采用沉积法固定化α-淀粉酶,并对固定条件进行优化。分别采用NaOH-CH2ClCOOH法和HNO3-H2SO4法制备羧基化氧化石墨烯,并将其用于固定化α-淀粉酶。结果表明,沉积法固定化酶的最适宜温度为65 ℃,最适宜pH=7.0。连续催化反应9次后,固定化酶活力仍能保持初始固定化酶活力的47.81%。经比较发现,NaOH-CH2ClCOOH法更有利于羧基化氧化石墨烯的制备,该方法制备的羧基化氧化石墨烯产率为HNO3-H2SO4法的1.2倍。
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| 关 键 词： | 催化剂工程 氧化石墨烯 沉积法 固定化酶 α-淀粉酶 羧基化氧化石墨烯 |
Immobilized α-amylase ongraphene oxide |
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| Zhang Han,Hua Shaofeng,Luo Hui,Zhang Lei,Fan Yunchang,Guo Fengzhe,Wei Dongyue,Zhang Meijin. Immobilized α-amylase ongraphene oxide[J]. Industrial Catalysis, 2018, 26(10): 92-96. DOI: 10.3969/j.issn.1008-1143.2018.10.013 |
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| Authors: | Zhang Han Hua Shaofeng Luo Hui Zhang Lei Fan Yunchang Guo Fengzhe Wei Dongyue Zhang Meijin |
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| Affiliation: | School of Chemistry & Chemical Engineering,Henan Polytechnic University,Jiaozuo 454000,Henan,China |
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| Abstract: | Graphite powder was used as raw material to prepare graphene oxide by improved Hummer method,and α-amylase was immobilized by deposition method and the immobilization conditions were optimized.The carboxylated graphene oxide was prepared by NaOH-CH2ClCOOH and HNO3-H2SO4,respectively,and used for immobilizing of α-amylase.Results showed that the optimum temperature for immobilization of the enzyme was 65 ℃ and the optimum pH was 7.0.After 9 consecutive using,activity for enzyme immobilization remained 47.81% of the initial activity.It was found that NaOH-CH2ClCOOH was more conducive for preparation of carboxylated graphene oxide.Yield of carboxylated graphene oxide prepared by NaOH-CH2ClCOOH was 1.2 times than by HNO3-H2SO4. |
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| Keywords: | catalyst engineering graphene oxide deposition immobilized enzyme alpha-amylase carboxylated graphene oxide |
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