稻壳固载焦化废水优势反硝化菌制备条件优化

选用从焦化废水的污泥中筛选出5株优势反硝化菌,采用固态培养技术将优势反硝化菌固定至稻壳载体.实验通过响应面法,以TTC脱氢酶酶活为响应值,对反硝化菌的固态培养条件：培养时间、培养温度、接种量、含水率进行优化;并研究3种保存方法对固载菌株TTC-脱氢酶活性的影响.实验结果表明,固态培养条件的最佳值：发酵时间为70h,培养温度为24.2℃,接种量为10.6/20,含水率为50.1%,固载菌株TTC-脱氢酶活性最大达到31.809（ugTF/g/h）;冷冻保存法对酶活影响最小,鼓风风干最经济实用.保存30d后的固载菌株48h对焦化废水的降解率达70%以上.

Optimization of preparation conditions for rice husk immobilized denitrifying bacteria from coking wastewater

To strengthen the microbial immobilization technology in the coking wastewater applications,five advantage denitrifying bacterias from the sludge of coking wastewater were selected.The advantage denitrifying bacteria were fixed on the rice husk containing coking wastewater to prepare immobilization products by using solid state fermentation technology.The TTC dehydrogenises activity were measured as an indicator for the solid-state fermentation of biomass,and then the fermentation conditions of denitrifying bacteria were optimized by the response surface methods,and the three preservation methods of fixed products：cryopreservation,blast air-dried were compared.The results show that：The main influential factors and the optimum values for solid-state fermentation are fermentation time of 70 h,fermentation temperature of 24.2 ℃,inoculation amount of 10.6/20 and moisture content of 50.1%.The maximum activity of immobilized products is 31.809（ugTF/g/h）,Cry-preservation is the method with minimal impact on the activity,the most economical and practical method is Cry preservation method.