Functional analysis of genes encoding putative oxidoreductases in Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase |
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Authors: | Yoshida Nobuyuki Akazawa Shin-Ichi Karino Tetsuya Ishida Hiroki Hata Yoji Katsuragi Tohoru Tani Yoshiki Takagi Hiroshi |
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Affiliation: | Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp |
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Abstract: | We found 11 genes (FAO1-11) encoding putative oxidoreductases in the Aspergillus oryzae genome, which are similar to fungal fructosyl-amino acid oxidases. The cDNAs corresponding to the genes were cloned and expressed in Escherichia coli. rFao2 had fructosyl-amino acid oxidase activity, whereas rFao1 did not show any enzyme activity, even though the deduced amino acid sequence of Fao1 is identical to that of one of the fructosyl-amino acid oxidase isozymes from Aspergillus oryzae. rFao7 and rFao8 showed oxidase activity toward sarcosine, L-pipecolate, and L-proline. rFao10 was active toward only sarcosine, of the substrates tested. The functions of the other proteins were also predicted from a phylogenetic analysis. |
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