| 三种金属离子协同复合酶制剂对木薯酒精产率的影响 |
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| 引用本文： | 王晨,马文勤,纪钧麟,郑鑫,贾正燕,夏迎春,尹芳,张无敌. 三种金属离子协同复合酶制剂对木薯酒精产率的影响[J]. 酿酒, 2020, 47(1): 72-76 |
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| 作者姓名： | 王晨 马文勤 纪钧麟 郑鑫 贾正燕 夏迎春 尹芳 张无敌 |
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| 作者单位： | 云南师范大学能源与环境科学学院(太阳能研究所),云南昆明,650500 |
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| 基金项目： | 2017年地方高校国家级大学生创新创业训练计划项目（201710681019）;国家自然科学基金（51366015）联合资助 |
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| 摘 要： | 为探究金属离子协同复合酶制剂对木薯原料产酒精的影响,选取了Mg2+、Zn2+和Fe3+结合纤维素酶和普鲁兰酶的复合酶制剂进行实验。实验结果表明Mg2+、Zn2+和Fe3+的最适添加量分别为0.8mg/g原料、1.2mg/g原料和0.4mg/g原料。其中Zn2+可以激活和促进发酵过程中的多种酶,效果优于Mg2+和Fe3+,是纤维素酶和普鲁兰酶组成的复合酶制剂的最适辅助金属离子,最适添加量为1.2mg/g原料,出酒率提高了8.44%。
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| 关 键 词： | 金属离子 复合酶制剂 木薯 酒精发酵 |
Effect of Three Metal Ions Synergistic Complex Enzyme Preparations on the Yield of Cassava Alcohol |
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| WANG Chen,MA Wenqin,JI Junlin,ZHENG Xin,JIA Zhengyan,XIA Yingchun,YIN Fang,ZHANG Wudi. Effect of Three Metal Ions Synergistic Complex Enzyme Preparations on the Yield of Cassava Alcohol[J]. Liquor Making, 2020, 47(1): 72-76 |
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| Authors: | WANG Chen MA Wenqin JI Junlin ZHENG Xin JIA Zhengyan XIA Yingchun YIN Fang ZHANG Wudi |
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| Affiliation: | (Yunnan Normal University School of Energy and Environment Science(Solar Energy Research Institute),Kunming 650500,China) |
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| Abstract: | In order to explore the effect of metal ion synergistic complex enzyme preparation on the production of alcohol in cassava raw materials,this study selected Mg2+,Zn2+and Fe3+combined with cellulase and pullulanase enzyme enzyme preparations for experiments.The experimental results showed that the optimum addition amount of Mg2+,Zn2+and Fe3+was 0.8mg/g raw material,1.2mg/g raw material and 0.4mg/g raw material.Among them,Zn2+can activate and promote various enzymes in the fermentation process,and the effect is better than Mg2+and Fe3+.It is the most suitable auxiliary metal ion for the complex enzyme preparation composed of cellulase and pullulanase.The optimum addition amount is 1.2mg/g raw materials,the rate of wine increased by 8.44%. |
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| Keywords: | Metal ions Complex enzyme preparation Cassava Alcohol fermentation |
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