实时荧光定量PCR技术在白酒酿造过程中微生物数量检测的应用展望

实时荧光定量PCR(real-time fluorescent quantitative polymerase chain reaction)是将普通PCR和光谱分析、实时检测等手段巧妙结合应用的一项技术。具有特异性强、灵敏度高、重复性好、定量准确、自动化程度高等优点,已成为分子生物学领域的重要技术工具。尤其是在微生物检测方面得到极大的推广应用。白酒酿造过程中微生物种类复杂、数量在不断变化,可培养方法的局限性很大,但是采用荧光定量PCR的方法有较理想的效果。本文对荧光定量PCR的原理、扩增序列的选择、基因组提取方法等方面做一综述。

Application of Real-Time Fluorescent Quantitative PCR for Monitoring Microbes in the Production of Baijiu

Real-time fluorescent quantitative polymerase chain reaction is a technology combining common PCR with spectral analysis and real-time detection.With its high specificity,high sensitivity,good repeatability,high accuracy and high degree of automation,RQ-PCR has become an important technical tool in the field of molecular biology,especially in microbe detection.Microbes involved in the production of Baijiu vary greatly in species and number,which limited the use of microbial culture method in the analysis of the microbes,while RQ-PCR may achieve ideal results.In this paper,the principle of RQ-PCR,the selection of amplified sequence,and the genome extraction methods were reviewed.