云烟87烤烟品种突变株的离体快繁技术研究

以云烟87烤烟品种突变巨型株的休眠芽为试材，对其进行了打破休眠促使萌芽、愈伤组织诱导、丛生芽增殖和生根培养的研究。结果表明:打破休眠促使其萌芽的最佳培养基配方为MS+2.0mg/L 6-BA+1.0mg/L NAA；诱导产生愈伤组织效果最佳的培养基配方为MS+3.0mg/L 6-BA+0.3mg/L NAA；丛生芽增殖效果最佳的培养基为MS+1.0mg/L 6-BA+0.3mg/L NAA；MS+0.6mg/L NAA对根系的生长最为有利。 

The Study on Vitro Rapid Propagation Technique of Yunyan 87 Flue-cured Tobacco Variety

With Yunyan 87 flue-cured mutant giant strains of dormant buds as test material, the breaking of dormancy to germination, callus induction, shoot proliferation and rooting culture were researched. The results showed that: the optimum culture medium for breaking of dormancy to was MS+2.0mg/L 6-BA+1.0mg/L NAA; the best medium formulation for callus induction was MS+3.0mg/L 6-BA+0.3mg/L NAA; the medium with optimal shoot proliferation effect was MS+1.0mg/L 6-BA+0.3mg/L NAA ; MS+0.6mg/L NAA was most on root growth .