| 聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性 |
| |
| 引用本文： | 谭帼馨,欧阳孔友,周 蕾,刘 燕,张 兰,宁成云.聚多巴胺螯合钙离子对钛表面的修饰及修饰后的细胞相容性[J].无机材料学报,2015,30(10):1075-1080. |
| |
| 作者姓名： | 谭帼馨 欧阳孔友 周 蕾 刘 燕 张 兰 宁成云 |
| |
| 作者单位： | (1. 广东工业大学 轻工化工学院, 广州510006; 2. 华南理工大学 材料科学与工程学院, 广州510641; 3. 西安交通大学 金属材料国家重点实验室, 西安710049) |
| |
| 基金项目： | 国家973计划项目(2012CB619100);国家自然科学基金(51372087);广东省科技计划项目(2014A010105048);广东省自然科学基金(2015A030313493);西安交通大学金属材料强度国家重点实验室开放研究项目(20141607) |
| |
| 摘 要： | 通过聚多巴胺自组装在钛表面构建钙离子螯合平台, 探讨聚多巴胺的酚羟基(-C-OH)和醌基(-C=O)螯合钙离子机理, 并研究其生物学性能。利用X射线光电子能谱分析聚多巴胺螯合钙离子化学基团的变化, 结果显示: 聚多巴胺螯合钙离子后, -C-OH峰面积从73.8%降至37.3%, -C=O从26.2%升至62.7%, 证明聚多巴胺的酚羟基与钙离子螯合过程中向醌基转化; 场发射扫描电镜、傅立叶红外光谱、X射线衍射仪和透射电镜分析表明聚多巴胺和钙离子的协同作用促进羟基磷灰石(HA)前驱体成核, 诱导HA的形成; 采用小鼠成骨细胞(MC3T3-E1)粘附和活性实验进行细胞相容性评价, 结果表明钛表面通过聚多巴胺螯合低浓度的钙离子有利于成骨细胞粘附, 且具有良好的细胞相容性。
|
| 关 键 词： | 聚多巴胺 螯合机理 钙离子 生物活性 细胞相容性 |
| 收稿时间： | 2015-02-11 |
| 修稿时间： | 2015-04-09 |
Titanium Modification by Calcium Ion Chelated Polydopamine and Its Cytocompatibility |
| |
| TAN Guo-Xin,OUYANG Kong-You,ZHOU Lei,LIU Yan,ZHANG Lan,NING Cheng-Yun.Titanium Modification by Calcium Ion Chelated Polydopamine and Its Cytocompatibility[J].Journal of Inorganic Materials,2015,30(10):1075-1080. |
| |
| Authors: | TAN Guo-Xin OUYANG Kong-You ZHOU Lei LIU Yan ZHANG Lan NING Cheng-Yun |
| |
| Affiliation: | (1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; 2. College of Materials Science and Technology, South China University of Technology, Guangzhou 510641, China; 3. State Key Laboratory for Mechanical Behavior of Materials, Xi’an Jiaotong University, Xi’an 710049, China) |
| |
| Abstract: | Calcium ions chelation platform was successfully obtained on a titanium surface by modification with polydopamine. The chelation mechanism of polydopamine with Ca2+ was studied. The in vitro bioactivity and cytocompatibility of Ca2+ chelation platform was assessed by incubation in simulated body fluids (SBF) and cultural osteoblast cells (MC3T3-E1), respectively. The results showed that the functional groups of polydopamine chelated Ca2+ were changed from phenolic hydroxyl to benzoquinone. The titanium surface modified by Ca2+-chelated polydopamine could induce nucleation and growth of hydroxyapatite in SBF. Meanwhile, Cell attachment and viability assay demonstrated that low concentration of Ca2+ chelation possessed good cytocompatibility. |
| |
| Keywords: | polydopamine chelation mechanism calcium ion bioactivity cytocompatibility |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《无机材料学报》浏览原始摘要信息 |
|
点击此处可从《无机材料学报》下载全文 |
|
