现场快速检测大田软海绵酸的传感器与仪器的研究 |
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引用本文: | 邱先鑫,苏凯麒,田玉兰,邹瞿超,方佳如,李蓉,王平. 现场快速检测大田软海绵酸的传感器与仪器的研究[J]. 传感技术学报, 2016, 29(5): 633-636. DOI: 10.3969/j.issn.1004-1699.2016.05.001 |
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作者姓名: | 邱先鑫 苏凯麒 田玉兰 邹瞿超 方佳如 李蓉 王平 |
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作者单位: | 浙江大学生物传感器国家专业实验室,生物医学工程教育部重点实验室,生仪学院,杭州310027;中科院传感技术国家重点实验室,上海200050;浙江大学生物传感器国家专业实验室,生物医学工程教育部重点实验室,生仪学院,杭州310027 |
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基金项目: | 国家海洋公益专项项目(201305010),教育部博士点基金项目(20120101130011),国家自然科学基金项目(31571004) |
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摘 要: | 研究并建立针对大田软海绵酸OA(okadaic acid)快速、有效的酶联免疫吸附试验ELISA(Enzyme-Linked Immunosorbent Assay)定量检测方法,制备了定量检测试剂盒-生物酶传感器,并研发配套的现场快速检测仪器-仿生电子眼(Bionic e-Eye),实现对OA的快速检测.对标准样品的参数进行测定,该OA试剂盒的检测限为0.19μg/L,检测范围为0.2μg/L~5μg/L;样品回收率为75%~125%,精密度在±15%以内.将Bionic e-Eye的检测结果和酶标仪及高效液相色谱HPLC(high performance liquid chromatog-raphy)所测数据的结果进行了比较,结果表明,Bionic e-Eye的性能与酶标仪的检测结果一致.此外,该仪器可以直接得出检测结果,不需要人工计算处理,适用于现场的快速检测,将是一种新的现场快速定量检测OA的技术手段.
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关 键 词: | 生物传感器 大田软海绵酸 仿生电子眼 试剂盒 ELISA |
The research of biosensor and Bionic e-Eye for rapid detection of okadaic acid on-site |
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Abstract: | Based onEnzyme-linked immunosorbent assay(ELISA),test kit,namely,enzyme sensor and the corre-sponding instrument was prepared,Bionic e-Eye for rapid,effective and quantitative detection of okadaic acid(OA) on-site. The detection limit of the kit is 0.19μg/L and the range is among 0.2μg/L and 5μg/L. The recovery rate is among 75%and 125%and the accuracy is within±15%. The performance of Bionic e-Eye is consistent with micro-plate reader and high performance liquid chromatography(HPLC). In addition,without artificial calculation,the instrument can show the results directly on the screen,which is very suitable for rapid detection,and will be a new technology for rapid and quantitative detection of OA on site. |
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Keywords: | Biosensor Okadaic Acid Bionic e-Eye Kit ELISA |
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