| 转基因油菜筛查检测策略研究 |
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| 引用本文： | 张丽,武玉花,吴刚,曹应龙,李均,卢长明.转基因油菜筛查检测策略研究[J].中国油料,2012(1):74-81. |
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| 作者姓名： | 张丽 武玉花 吴刚 曹应龙 李均 卢长明 |
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| 作者单位： | 中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,湖北武汉,430062 |
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| 基金项目： | 转基因生物新品种培育重大专项（2009ZX08012-009B;2011ZX08012-003;2011ZX08012-005） |
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| 摘 要： | 根据收集到的国内外已商业化转基因油菜品种的基因元件信息,构建了油菜转基因检测分子特征矩阵,在分析基因元件使用频率的基础上,建立了转基因油菜的筛查检测策略。研究发现,利用P-CaMV35S、P-FMV35S和bar这三个元件组合,对现有转基因油菜品种的筛查检出率可达100%。将P-CaMV35S、P-FMV35S、bar、CP4-EPSPS、PAT和T-NOS组合,每个转化事件可检出至少两次,增加了检测的可靠性。同时,分析了不同转化事件中同名基因元件的序列差异,进一步验证了分子特征矩阵筛查方法的可行性。
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| 关 键 词： | 转基因油菜 转基因检测 筛查方法 矩阵分析 PCR |
Strategy of transgenic rapeseed screening based on exogenous gene elements |
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| Authors: | ZHANG Li WU Yu-hua WU Gang CAO Ying-long LI Jun LU Chang-ming |
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| Affiliation: | (Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | Based on inserted gene elements information for commercial domestic and foreign events in transgenic rapeseed,a matrix of molecular characteristics from transgenic rapeseed was established to investigate the gene frequencies.A screening strategy was developed for detecting complex molecular structure in transgenic rapeseed events based on element frequencies.Results showed that the detection rate could reach 100% for existing transgenic rapeseed events using the combination of P-CaMV35S,P-FMV35S and bar.When using element set of P-CaMV35S,P-FMV35S,bar,CP4-EPSPS,PAT and T-NOS,every transgenic event was detected more than once.Sequence alignments of the synonymous elements used in transgenic events were also analysed which improved the reliability of the matrix screening detection method. |
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| Keywords: | Genetically modified rapeseed GMO detection Screening method Matrix analysis PCR |
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