| 橘林油脂酵母发酵条件的初步研究 |
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| 引用本文： | 张东辉,梁焯,万霞,龚阳敏,江木兰. 橘林油脂酵母发酵条件的初步研究[J]. 中国油料, 2012, 0(1): 82-86,95 |
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| 作者姓名： | 张东辉 梁焯 万霞 龚阳敏 江木兰 |
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| 作者单位： | [1]中国农业科学院油料作物研究所,湖北武汉,430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉,430062 |
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| 基金项目： | 国家863计划（2011AA100904）; 国家自然科学基金（30970262） |
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| 摘 要： | 以橘林油脂酵母（Lipomyces kononenkoae CICC1714）为实验材料,通过设计单因素实验,确定了摇瓶发酵培养的最佳产油条件为：氮源为硫酸铵,碳源为葡萄糖,培养温度28℃,接种量10%,初始pH值为7.0,最适碳氮比为99。优化后批次发酵的生物量为7.01g/L,油脂含量15.91%,油脂产量1.12g/L。经半连续发酵后最终的生物量为24.13g/L,油脂产量为3.06g/L。
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| 关 键 词： | 橘林油脂酵母 单因素实验 发酵条件 微生物油脂 |
Investigation of fermentation conditions for microbial oil production by Lipomyces kononenkoae |
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| ZHANG Dong-hui,LIANG Zhuo,WAN Xia,GONG Yang-min,JIANG Mu-lan. Investigation of fermentation conditions for microbial oil production by Lipomyces kononenkoae[J]. , 2012, 0(1): 82-86,95 |
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| Authors: | ZHANG Dong-hui LIANG Zhuo WAN Xia GONG Yang-min JIANG Mu-lan |
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| Affiliation: | 1.Oil Crops Research Institute of Chinese Academy of Agriculture Sciences,Wuhan 430062,China; 2.Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China) |
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| Abstract: | The oil-producing conditions of Lipomyces kononenkoae were optimized on flask fermentation level by the single-factor experiment.The highest lipid yield was obtained with ammonium sulfate and glucose as nitrogen source and carbon source,respectively.The optimum culture conditions for temperature,inoculation,initial pH value and C/N ratio were 28℃,10%,7.0 and 99,respectively.Batch culture result showed that the biomass,lipid content and lipid yield of Lipomyces kononenkoae could reach 7.01g/L,15.91%,1.12g/L respectively after optimization.The results of flask fed-batch cultures indicated the dry biomass and lipid yield of Lipomyces kononenkoae could further reach 24.13g/L and 3.06g/L respectively. |
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| Keywords: | Lipomyces kononenkoae Single-factor experiment Fermentation condition Microbial oil |
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