| 尖孢镰刀菌胡麻专化型（Fusarium oxysporum f.sp.lini）ISSR标记聚类分析 |
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| 引用本文： | 苑琳,刘姗姗,路福平,李子钦,张辉,陈佩文.尖孢镰刀菌胡麻专化型（Fusarium oxysporum f.sp.lini）ISSR标记聚类分析[J].中国油料,2012(2):193-200. |
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| 作者姓名： | 苑琳 刘姗姗 路福平 李子钦 张辉 陈佩文 |
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| 作者单位： | [1]天津科技大学生物工程学院工业微生物重点实验室,天津300457 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [3]内蒙古农牧业科学院,内蒙古呼和浩特010031 [4]内蒙古师范大学田家炳教育书院,内蒙古呼和浩特010022 |
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| 基金项目： | 国家油用胡麻产业技术体系专项（CARS-17） |
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| 摘 要： | 结合形态学特征和分子生物学方法对我国尖孢镰刀菌胡麻专化型菌株进行鉴定、聚类分析和遗传变异分析。结合传统分类方法和ITS序列分析,确定6省区96株菌株为尖孢镰刀菌。采用ISSR（简单重复序列区间）分子标记技术对这96株菌株进行分析,12条引物共扩增出800个条带,多态率条带数为797条,多态率为99.62%;在相似性系数为0.88处,96株供试菌株被分为5个类群。聚类结果表明,胡麻枯萎病菌种内存在明显的多态性,且ISSR类群与地理来源存在相关性。
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| 关 键 词： | 尖孢镰刀菌胡麻专化型 ISSR（简单重复序列区间） 聚类分析 ITS |
Cluster analysis by ISSR markers on Fusarium oxysporum f.sp.lini |
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| Authors: | YUAN Lin LIU Shan-shan LU Fu-ping LI Zi-qin ZHANG Hui CHEN Pei-wen |
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| Affiliation: | 1.Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;2.College of Life Science,Inner Mongolia University, Hohhot 010021,China;3.Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031,China;4.College of Inner Mongolia Normal University,Hohhot 010022,China) |
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| Abstract: | Cluster analysis on Fusarium oxysporum f.sp.lini was carried out using ISSR（inter-simple sequence repeat） markers.According to morphology feature and ITS sequence features,120 isolates from 6 provinces were identified,and 96 were Fusarium oxysporum.Then the 96 strains were clustered by ISSR markers.A total of 800 bands were amplified with 12 primers,in which 797 bands were polymorphous（about 99.62%）.The 96 strains were clustered into 5 groups at 0.88.Results indicated that there were rich polymorphism among Fusarium oxysporum f.sp.lini strains.Cluster groups also indicated relevance among their geographical origins. |
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| Keywords: | Fusarium oxysporum f sp lini ISSR Cluster analysis ITS |
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