| 氮源对利迪链菌素生产及相关次级代谢物分布的影响 |
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| 引用本文： | 李良智,乔斌,元英进.氮源对利迪链菌素生产及相关次级代谢物分布的影响[J].中国化学工程学报,2007,15(3):403-410. |
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| 作者姓名： | 李良智 乔斌 元英进 |
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| 作者单位： | Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 30072, China |
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| 基金项目： | 国家自然科学基金，教育部长江学者和创新团队发展计划 |
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| 摘 要： | The effects of nitrogen sources on streptolydigin production and distribution of secondary metabolites were investigated for flask cultured S.lydicus AS 4.2501.When peptone,asparamide,and glutamic acid were ex- amined as the nitrogen source,respectively,liquid chromatography-mass spectrometry（LC-MS）and photodiode array（PDA）analyses revealed the formation of two analogues of streptolydigin in the fermentation broth.When soybean meal was used as the source of nitrogen,three analogues of streptolydigin were detected.The use of am- monium sulfate as a source of nitrogen resulted in a lower pH value of the fermentation system,thus inhibiting streptolydigin biosynthesis and changing the metabolic profiling.Among the nitrogen sources that were made use of,glutamic acid was most favorable to the formation of streptolydigin.Simultaneously,this study also showed that the changing nitrogen sources resulted in altering the production and relative ratios of streptolydigin and its analogues.
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| 关 键 词： | 氮源 利迪链菌素 生产 次级代谢物 分布 |
| 收稿时间： | 31 August 2006 |
| 修稿时间： | 2006-08-312007-02-12 |
Nitrogen sources affect streptolydigin production and related secondary metabolites distribution of Streptomyces lydicus AS 4.2501 |
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| LI Liangzhi,QIAO Bin,YUAN Yingjin.Nitrogen sources affect streptolydigin production and related secondary metabolites distribution of Streptomyces lydicus AS 4.2501[J].Chinese Journal of Chemical Engineering,2007,15(3):403-410. |
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| Authors: | LI Liangzhi QIAO Bin YUAN Yingjin |
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| Affiliation: | Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 30072, China;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 30072, China;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 30072, China |
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| Abstract: | The effects of nitrogen sources on Streptolydigin production and distribution of secondary metabolites were investigated for flask cultured S.lydicus AS 4.2501.When peptone, asparamide, and glutamic acid were examined as the nitrogen source, respectively, liquid chromatography-mass spectrometry (LC-MS) and photodiode array (PDA) analyses revealed the formation of two analogues of Streptolydigin in the fermentation broth.When soybean meal was used as the source of nitrogen, three analogues of Streptolydigin were detected.The use of ammonium sulfate as a source of nitrogen resulted in a lower pH value of the fermentation system, thus inhibiting Streptolydigin biosynthesis and changing the metabolic profiling.Among the nitrogen sources that were made use of, glutamic acid was most favorable to the formation of Streptolydigin.Simultaneously, this study also showed that the changing nitrogen sources resulted in altering the production and relative ratios of Streptolydigin and its analogues. |
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| Keywords: | Streptomyces lydicus nitrogen source analogue secondary metabolite Streptolydigin |
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