| 阿里红多糖组分对RAW264.7巨噬细胞免疫功能的影响 |
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| 引用本文： | 祖丽胡玛尔?阿卜杜合力力,帕丽达?阿不力孜,木尼萨?迪力夏提,依丽努尔?艾尼瓦尔,丛媛媛,米仁沙?牙库甫.阿里红多糖组分对RAW264.7巨噬细胞免疫功能的影响[J].食品安全质量检测技术,2017,8(12):4638-4644. |
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| 作者姓名： | 祖丽胡玛尔?阿卜杜合力力 帕丽达?阿不力孜 木尼萨?迪力夏提 依丽努尔?艾尼瓦尔 丛媛媛 米仁沙?牙库甫 |
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| 作者单位： | 新疆医科大学药学院,新疆医科大学药学院,新疆医科大学药学院,新疆医科大学药学院,新疆医科大学药学院,新疆医科大学药学院 |
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| 基金项目： | 国家自然科学基金项目(81460635)、新疆维吾尔自治区科技支疆项目(201591190) |
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| 摘 要： | 目的研究阿里红多糖(Fomes Officinals polysaccharide,FOPS)及其组分FOPS-a、FOPS-b对RAW264.7巨噬细胞的免疫调节作用。方法以小鼠RAW264.7巨噬细胞为研究对象,不同浓度(50~1600μg/m L)FOPS、FOPS-a、FOPS-b干预后,用cck-8试剂盒测定细胞活力,中性红法测定其吞噬活性,NO试剂盒测定NO的含量,ELISA试剂盒测定TNF-α和IL-1β释放能力。结果 FOPS、FOPS-a、FOPS-b在一定的浓度范围内可以提高RAW264.7巨噬细胞的增殖活性、吞噬活性、一氧化氮以及TNF-α、IL-1β的释放量。结论 FOPS、FOPS-a、FOPS-b一定浓度范围内具有良好的增强RAW264.7巨噬细胞免疫功能的作用。
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| 关 键 词： | 阿里红多糖 RAW264.7巨噬细胞 免疫活性 |
| 收稿时间： | 2017/8/21 0:00:00 |
| 修稿时间： | 2017/12/9 0:00:00 |
Effects of Fomes Officinals polysaccharide on immune function of RAW264.7 macrophage cell |
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| ZULIHUMAER?Abuduhelili,PALIDA?Abulizi,MUNISA?Dilixiati,YILINUER?Ainiwaer,CONG Yuan-Yuan and MIRENSHA?Yakufu.Effects of Fomes Officinals polysaccharide on immune function of RAW264.7 macrophage cell[J].Food Safety and Quality Detection Technology,2017,8(12):4638-4644. |
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| Authors: | ZULIHUMAER?Abuduhelili PALIDA?Abulizi MUNISA?Dilixiati YILINUER?Ainiwaer CONG Yuan-Yuan and MIRENSHA?Yakufu |
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| Affiliation: | College of Pharmacy, Xinjiang Medical University,College of Pharmacy, Xinjiang Medical University,College of Pharmacy, Xinjiang Medical University,College of Pharmacy, Xinjiang Medical University,College of Pharmacy, Xinjiang Medical University and College of Pharmacy, Xinjiang Medical University |
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| Abstract: | Objective To investigate the effects of Fomes Officinals polysaccharide (FOPS), FOPS-a and FOPS-b on the immune function of RAW264.7 macrophage cells. Methods RAW264.7 macrophage cell were taken as the research object and the effects of FOPS, FOPS-a and FOPS-b on cell proliferation, phagocytosis of RAW264.7 cells, levels of NO, TNF-a, IL-1? production were detected by cck-8 kit, neutral red test, NO kit and ELISA kit, respectively. Results In certain range of concentration, the cell proliferation ability, the phagocytosis of RAW264.7 cells and the production of NO, TNF-a, IL-1? in macrophages were increased. Conclusion FOPS, FOPS-a and FOPS-b can enhance the immune functions of RAW264.7 macrophage in a certain concentration range. |
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| Keywords: | Fomes Officinals polysaccharide RAW264 7 macrophage cell immune function |
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