| 高效液相色谱法同时测定粮食中6种大豆异黄酮 |
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| 引用本文： | 胡莉,靳可婷,仲伶俐,杨晓凤,江永洪. 高效液相色谱法同时测定粮食中6种大豆异黄酮[J]. 食品安全质量检测学报, 2017, 8(11): 4368-4374 |
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| 作者姓名： | 胡莉 靳可婷 仲伶俐 杨晓凤 江永洪 |
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| 作者单位： | 四川省农业科学院分析测试中心, 农业部农产品质量安全风险评估实验室(成都), 农业部食品质量监督检验测试中心(成都),四川省农业科学院分析测试中心, 农业部农产品质量安全风险评估实验室(成都), 农业部食品质量监督检验测试中心(成都),四川省农业科学院分析测试中心, 农业部农产品质量安全风险评估实验室(成都), 农业部食品质量监督检验测试中心(成都),四川省农业科学院分析测试中心, 农业部农产品质量安全风险评估实验室(成都), 农业部食品质量监督检验测试中心(成都),四川省农业科学院分析测试中心, 农业部农产品质量安全风险评估实验室(成都), 农业部食品质量监督检验测试中心(成都) |
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| 基金项目： | 四川省财政基因工程专项资金(优秀论文基金)项目(2013LWJJ-012, 2014LWJJ-012) |
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| 摘 要： | 目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定粮食中6种大豆异黄酮(大豆苷、大豆黄苷、染料木苷、大豆素、大豆黄素和染料木素)含量的分析方法。方法准确称取一定量粉碎混匀后的样品,经80%甲醇提取后取上清液,过0.22μm有机相滤膜上机。采用Thermo Syncronis C18柱(250 mm×4.6 mm,5μm),以0.5%甲酸水溶液(A)、乙腈(B)和甲醇(C)作为流动相进行梯度洗脱,流速0.8m L/min,柱温为35℃,于紫外检测器波长260 nm处检测,大豆异黄酮各组分含量以外标法进行定量。结果本方法在30 min内完成6种大豆异黄酮的分离分析,大豆异黄酮各组分浓度在0.2~50μg/mL范围内呈良好的线性关系(r0.999),平均加标回收率为96.9%~107.8%,相对标准偏差(relative standard deviation,RSD)为0.6%~5.0%,检出限为0.03~0.1μg/mL,定量限为0.1~0.3μg/mL。结论建立的方法具有较高的灵敏度和重复性,能满足不同粮食种类中大豆异黄酮含量测定。
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| 关 键 词： | 高效液相色谱法 大豆异黄酮 粮食 |
| 收稿时间： | 2017-07-01 |
| 修稿时间： | 2017-11-02 |
Determination of 6 kinds of isoflavones in food crops by high performance liquid chromatography |
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| HU Li,JIN Ke-Ting,ZHONG Ling-Li,YANG Xiao-Feng and JIANG Yong-Hong. Determination of 6 kinds of isoflavones in food crops by high performance liquid chromatography[J]. Journal of Food Safety & Quality, 2017, 8(11): 4368-4374 |
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| Authors: | HU Li JIN Ke-Ting ZHONG Ling-Li YANG Xiao-Feng JIANG Yong-Hong |
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| Affiliation: | Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment or Agro-Product (Chengdu), Ministry of Agriculture, Food Quality Supervision and Inspection Center of Agricultural Ministry of China (Chengdu),Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment or Agro-Product (Chengdu), Ministry of Agriculture, Food Quality Supervision and Inspection Center of Agricultural Ministry of China (Chengdu),Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment or Agro-Product (Chengdu), Ministry of Agriculture, Food Quality Supervision and Inspection Center of Agricultural Ministry of China (Chengdu),Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment or Agro-Product (Chengdu), Ministry of Agriculture, Food Quality Supervision and Inspection Center of Agricultural Ministry of China (Chengdu) and Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Laboratory of Quality and Safety Risk Assessment or Agro-Product (Chengdu), Ministry of Agriculture, Food Quality Supervision and Inspection Center of Agricultural Ministry of China (Chengdu) |
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| Abstract: | |
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| Keywords: | high performance liquid chromatography isoflavones food crops |
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