固相萃取-高效液相色谱法测定果醋中7种有机酸

目的：建立果醋中7种有机酸同时测定的高效液相色谱方法。方法：用水提取果醋中有机酸，后经LC-SAX固相萃取柱净化，以Atlantis T3 C18色谱柱进行分离，二极管阵列检测器检测。结果：在优化色谱条件下，7种有机酸在15min内分离良好，各组分回收率在97.5%～105.1%，相对标准偏差不大于1.31%，最低检出限在0.30～1.80μg/mL范围。结论：该方法具有简便、快速、准确等优点，可应用于果醋中有机酸的测定。

Determination of Seven Organic Acids in Fruit Vinegar by Solid-phase Extraction and High Performance Liquid Chromatography

Objective: To develop an analytical method for the simultaneous determination of seven organic acids in fruit vinegar by high-performance liquid chromatography (HPLC). Methods: Organic acids in fruit vinegar were extracted with water, cleaned up using an LC-SAX solid phase extraction cartridge, separated using an Atlantis T3 C18 column and detected using a diode-array detector. Results: Seven organic acids were separated within 15 minutes. The recovery rates of seven organic acids were in the range of 97.5%－105.1% with RSD of less than 1.31%. The limits of detection were 0.30－1.80 μg/mL. Conclusion: This method provides a simple, rapid, accurate and suitable method for the simultaneous determination of organic acids in fruit vinegar.