大豆发芽过程中蛋白质的水解作用研究

研究大豆发芽过程中内源蛋白酶的蛋白水解作用,分析大豆种子吸水量、内源蛋白酶活性、发芽长度、游离氨基酸含量及发芽后大豆蛋白质亚基组成的变化。结果发现,大豆在发芽48h以后,大豆芽表现出快速的增长,其内源蛋白酶的活性达到最高,水解物游离氨基酸的含量变化规律与蛋白酶的活性变化规律一致。大豆发芽过程中,内源蛋白酶主要使11S大豆球蛋白的碱性亚基B3发生降解,对贮藏蛋白的主要成分7S和11S几乎没有作用。

Study on hydrolysis of protein during soybean germination

The study was on protein hydrolysis of endogenous protease during soybean germination.The change of soybean seed water absorbing capacity,endogenous protease vitality,sprout length,free amino acid content and soybean protein subunit fractions content were analyzed during soybean seed germination.The result showed that soybean sprout grew quickly and endogenous protease vitality reached up to the highest after 48h germination.The change rule of free amino acid content was the same with that of endogenous protease vitality.It was also found that it was basic subunit B3 of 11S glycinin that mainly degraded during the germination period and there was almost no degradation effect on 7S and 11S,main components in soybean storage protein.