肌红蛋白在阴极沉积的Fe3O4-PDDA膜上的固定及其电化学性质研究

利用阴极电解沉积方法在玻碳电极表面制备了具有生物兼容性的Fe3O4-PDDA杂化膜.这种膜对肌红蛋白(Mb)和血红蛋白(Hb)有很强的吸附能力,并能实现它们与电极表面的直接电子传输.采用X射线光电子能谱、原子力显微镜、紫外光谱、电化学阻抗谱和循环伏安等手段对沉积的复合物膜进行了表征,并研究了Mb和Fe3O4-PDDA之间的相互作用力.这种阴极电解沉积制备氧化物-聚合物膜的方法操作简单快捷,可在聚合物基质中现场合成功能性的氧化物膜.因此,在制备性能优良的固定蛋白质的基体材料和制备传感器件方面有很好的应用前景.

Electrochemistry of myoglobin adsorbed in cathodically deposited Fe304-Poly(diallyldimethylammonium chloride)film

A good biocompatible Fe3O4-PDDA poly(diallyldimethylammonium chloride) membrane has been prepared by a novel cathodically electrolytic deposition method. This method combined the advantages of simplification,convenience,rapidness and the in-situ synthesis of Fe3O4 and introduction of polymers for preventing aggregation. The obtained homogeneous film has strong adsorption ability for protein and could greatly facilitate the direct electron transfer of immobilized myoglobin (Mb) and hemoglobin (Hb),which is resulted from the cooperation of PDDA and Fe3O4 nanoparticles. Many techniques,such as X-ray photoelectron spectroscopy (XPS),atomic force microscope (AFM),electrochemical impedance spectroscopy (EIS) and cyclic voltammetry were used to characterize the film and study the interactions between Mb and Fe3O4-PDDA. This presented system supplied an attractive prospect to prepare novel metal oxide-polyelectrolyte hybrid films for studying proteins' electrochemical behaviors and fabricating many other enzyme biosensors due to the versatile affinity of the film for the biomolecular.