顶空进样-毛细管气相色谱法检测饮料用焦糖色素中的4-甲基咪唑

建立了顶空进样-毛细管气相色谱法测定饮料用焦糖色素中4-甲基咪唑含量的方法。采用HP-5毛细管柱、氮磷检测器，溶剂为二氯甲烷，内标为N，N-二甲基苯胺。方法的线性范围为0．025μg／ml-0．295μg／ml，最低检测浓度为0．01μg/ml；回收率在99％以上，相对标准偏差在2％之内。该方法重现性好、简单、快速、准确，能满足分析检测的需要。

Determination of 4-methylimidazole in caramel pigment used in beverages by capillary GC with headspace injection

A capillary gas chromatography with headspace injection was established for the determination of 4-methylimidazole in caramel pigment used in beverages. An HP-5 capillary column and nitrogen phosphorus were used with methylene chloride as the solvent and N, N-dimethylbenzenamine as the internal standard. The linear range was 0.025 μg/ml - 0.295 μg/ml and the limit of detection 0.01 μg/ml. The average recoveries were above 99% and the relative standard deviation within 2%. Featured by good reproducibility, the method was simple, rapid and sensitive enough to meet the needs of analysis and determination.