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Effect of bacterial alginate on growth of mesenchymal stem cells |
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| Authors: | Elizaveta Akoulina Andrej Dudun Anton Bonartsev Garina Bonartseva Vera Voinova |
| Affiliation: | 1. Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia;2. Research Center of Biotechnology RAS, Moscow, Russiaakoulinaliza@gmail.com;4. Research Center of Biotechnology RAS, Moscow, Russia;5. Research Center of Biotechnology RAS, Moscow, Russia |
| Abstract: | AbstractCapsular alginate was synthesized using bacteria Azotobacter agile 12 in order to test its potential for use in biotechnology and tissue engineering. Capsular bacterial alginate was isolated and purified using EDTA treatment and dialysis. Calcium alginate spheres were produced and their effect on growth of mesenchymal stem cells was studied. Bacterial alginate shows significantly lower cytotoxicity than commercial alginate isolated from algae. |
| Keywords: | Azotobacter bacterial alginate capsular alginate cytotoxicity MSC |