Aflatoxins in cotton seeds and cotton seed cake from Pakistan |
| |
| Authors: | Z H Shar Om Pirkash H H Shar S T H Sherazi S A Mahesar |
| |
| Affiliation: | 1. Dr. M.A Kazi Institute of Chemistry University of Sindh, Jamshoro, Pakistanzahidshar_2009@yahoo.com;3. Dr. M.A Kazi Institute of Chemistry University of Sindh, Jamshoro, Pakistan;4. National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan |
| |
| Abstract: | ABSTRACTA study was conducted to evaluate the natural occurrence of aflatoxins (AFB1, AFB2, AFG1, AFG2) in cotton seeds (n = 110) and cotton seed cake (CSC; n = 110) from Pakistan. All samples were screened by Enzyme-Linked ImmunoSorbent Assay (ELISA). Positive samples were further quantified by IAC-HPLC-FLD. Total contamination frequency and aflatoxins mean levels were 80% and 69 μg/kg in cotton seeds and the corresponding values for cotton seed cake 88% and 89 μg/kg, respectively. Aflatoxin B1was found in all positive samples and co-occurred with AFB2, AFG1, and AFG2. Sixty-four cotton seeds and 71 CSC samples contained aflatoxins levels higher than the ML set for animal feed (20 µg/kg). The results of the present study will help the regulatory authorities to formulate strategies for monitoring aflatoxins in animal feeds. |
| |
| Keywords: | Aflatoxins cotton seeds cotton seed cake HPLC animal feed |
|
|
