周期变温和后期通氮气或二氧化碳发酵对提高甘油产量的影响

引　言甘油是一种重要的工业原料 ,广泛用于化工、食品、医药等领域 .生产甘油的方法主要有化学合成、油脂水解和肥皂液提取以及糖类原料经微生物作用的发酵法 .由于化学合成甘油的产量有限 ,肥皂行业受到洗涤剂行业的冲击 ,肥皂生产减少 ,甘油产量也随之下降 ,市场供不应求 .因此 ,发酵法以其原料来源丰富、设备简单等得到人们的青睐1～ 3 ] .发酵法是以糖类为原料 ,利用某些微生物在一定条件下通过代谢作用而产生甘油 .用耐高渗酵母发酵生产甘油的研究已有许多文献进行了报道4～ 7] ,我国在这方面也做了大量的研究工作 .但大规模工业化…

EFFECTS OF OSCILLATORY TEMPERATURE AND SPARGING NITROGEN GAS OR CARBON DIOXIDE DURING LATER STAGE ON GLYCEROL FERMENTATION

Effects of periodic temperature variation on glycerol fermentation by osmophilic yeast was discussed.At the same time,glycerol fermentation by periodic temperature variation,constant temperature,aerating nitrogen or carbon dioxide during the later stage were compared.The result showed that glycerol yield by periodic temperature variation fermentation was higher than that by constant temperature fermentation and an appropriate temperature oscillatory range was 35-45℃.During the later stage of constant temperature fermentation,when fermentation time was 72h,glycerol yield was the highest.After that,glycerol yield began to reduce and glycerol began to be consumed.Then,nitrogen gas or carbon dioxide was sparged in fermentation medium to ferment glycerol unceasingly,which can decrease residual sugar,increase glycerol yield further and avoid glycerol being consumed.Moreover,during the later stage of glycerol fermentation,glycerol yield by sparging nitrogen gas was higher than that by sparging carbon dioxide.